The extracellular domain interacts with natriuretic peptides and triggers the intracellular guanylyl cyclase domain to convert GTP to cGMP. To effectively develop methods to regulate pGC-A, structural information on the full-length form is needed. However, structural data on the transmembrane and ...
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially
Body coloration is an ecologically important trait that is often involved in prey-predator interactions through mimicry and crypsis. Although this subject has attracted the interest of biologists and the general public, our scientific knowledge on the su
How a single cell ultimately gives rise to a patterned, complex organism is a fundamental question in biology. Embryonic development can be generalized as a process of progressive restriction of cellular potential. In vertebrates, the zygote is totipotent, but by blastula stages the three primary ge...
Rhabdomeric photoreceptors of eyes in the terrestrial slug Limax are the typical invertebrate-type but unique in that three visual opsins (Gq-coupled rhodopsin, xenopsin, Opn5A) and one retinochrome, all belonging to different groups, are co-expressed. H
Ferroptosis, a relatively recent discovery in the realm of cell biology, represents a unique form of regulated cell death. This process hinges on a distinct chemical cascade ignited by the iron-dependent buildup of lipid peroxides. Ferroptosis, in essence, is orchestrated by an intricate interplay...
Heterotrimeric G-proteins, consisting of three distinct subunits: Gα, Gβ and Gγ, constitute one of the most important signal transduction systems in eukaryotes. According to the classic paradigm, ligand-bound G-protein coupled receptors catalyze the exchange of GDP for GTP on the Gα subunit...
Sponges (phylum Porifera) constantly interact with microbes. They graze on microbes from the water column by filter-feeding and they harbor symbiotic partners within their bodies. In experimental setups, sponges take up symbionts at lower rates compared
On the one hand, the affinity of KRAS and GTP is at the pM level, while the concentration of GTP in cells is up to 0.5 μM. It is difficult to achieve effective competition like protein kinase inhibitors [18, 19]. On the other hand, the KRAS proteins are featureless. They have ...
Drought severely limits sunflower production especially at the seedling stage. To investigate the response mechanism of sunflowers to drought stress, we utilized two genotypes of sunflower materials with different drought resistances as test materials. T