L-ArabinoseD-XyloseGratuitous inducerα-GalactosidaseL -Arabinose has been described as a gratuitous inducer of the yeast 伪-galactosidase. This has been found to be an artefact resulting from galactose contamination of commercial samples of L -arabinose. The inactivation produced on UDP-glucose 4-...
From sequence analysis and an AlphaFold structural prediction of the complex between KdpDNTDand the KdpB A domain34,35, three conserved common ATP-binding motifs, characteristic for an ASK, are apparent: Walker A and Walker B motifs for coordination of the β- and γ-phosphates, and a trypto...
It should be noticed that there is a reported example of a sulfone glycoside, whose carbohydrate moiety is again arabinose59. The potential applications of O-xylosyl phosphates or O-xylosyl phosphonates are less clear. Despite the fact that sugar 1-phosphates are products with excellent ...
In these experiments, we used a strain in which ffh expression is under control of an arabinose-inducible promoter (WAM113). Transcription from this promoter can be tightly repressed, and WAM113 cells die in the absence of inducer (Phillips and Silhavy 1992). Insertion of the AP fusions was...
At anA 600 = 1.0, l-arabinose inducer was added to a final concentration of 0.1% (w/v) and shaking continued for an additional 3 h at 30 °C. Cells were harvested at 10,000 rpm for 10 min in a Sorvall GSA rotor and resuspended in a minimal volume of 50 mm Tris-HCl, pH 7.6,...
The increased digestibility of IL-pretreated substrates in the cultures and the corresponding accumulation of xylose and arabinose (Fig. 2) had a pronounced effect on the expression of CAZymes. The abundance of total CAZy transcripts in A. niger had a bi-modal pattern in the ILS cultures, wher...
The increased digestibility of IL-pretreated substrates in the cultures and the corresponding accumulation of xylose and arabinose (Fig. 2) had a pronounced effect on the expression of CAZymes. The abundance of total CAZy transcripts in A. niger had a bi-modal pattern in the ILS cultures, wher...
Plasmids allowing the production of AtcC with a 6His-tag and AtcB with a CBP tag, as well as control plasmids, were co-transformed in an E. coli strain and the proteins were produced by adding the arabinose inducer. We purified AtcB on calmodulin beads and observed on a western blot ...
Apart from the Leloir pathway and the oxidative pathway, there is an alternative oxido-reductive pathway. This oxido-reductive pathway has similarities to the metabolic pathway of l-arabinose, and in Trichoderma reesei (Hypocrea jecorina) and Aspergillus nidulans the same enzyme is employed for the ...
Apart from the Leloir pathway and the oxidative pathway, there is an alternative oxido-reductive pathway. This oxido-reductive pathway has similarities to the metabolic pathway of L-arabinose, and in Trichoderma reesei (Hypocrea jecorina) and Aspergillus nidulans the same enzyme is employed for the ...