L-ArabinoseD-XyloseGratuitous inducerα-GalactosidaseL -Arabinose has been described as a gratuitous inducer of the yeast 伪-galactosidase. This has been found to be an artefact resulting from galactose contamination of commercial samples of L -arabinose. The inactivation produced on UDP-glucose 4-...
Cultures were grown at 25 °C for 4 h post addition of the indicated concentrations of L-arabinose inducer. Only an L-arabinose concentration of 0.001% w/v resulted in exclusive overproduction of full length recombinant PfVIT correctly targeted to the E. coli inner membrane. Upper arrow ...
In these experiments, we used a strain in which ffh expression is under control of an arabinose-inducible promoter (WAM113). Transcription from this promoter can be tightly repressed, and WAM113 cells die in the absence of inducer (Phillips and Silhavy 1992). Insertion of the AP fusions was...
It should be noticed that there is a reported example of a sulfone glycoside, whose carbohydrate moiety is again arabinose59. The potential applications of O-xylosyl phosphates or O-xylosyl phosphonates are less clear. Despite the fact that sugar 1-phosphates are products with excellent ...
At anA 600 = 1.0, l-arabinose inducer was added to a final concentration of 0.1% (w/v) and shaking continued for an additional 3 h at 30 °C. Cells were harvested at 10,000 rpm for 10 min in a Sorvall GSA rotor and resuspended in a minimal volume of 50 mm Tris-HCl, pH 7.6,...
The pre-culture was added 1:100 to flasks containing 10 mL of Lauria Bertani medium that were initially incubated at 37°C in an orbital shaker. The temperature was lowered to 30, 25 or 20°C at a bacterial OD600 = 0.2 and the recombinant expression was induced by arabinose (1,5 mg/...
The pre-culture was added 1:100 to flasks containing 10 mL of Lauria Bertani medium that were initially incubated at 37°C in an orbital shaker. The temperature was lowered to 30, 25 or 20°C at a bacterial OD600 = 0.2 and the recombinant expression was induced by arabinose (1,5 mg/...
Apart from the Leloir pathway and the oxidative pathway, there is an alternative oxido-reductive pathway. This oxido-reductive pathway has similarities to the metabolic pathway of l-arabinose, and in Trichoderma reesei (Hypocrea jecorina) and Aspergillus nidulans the same enzyme is employed for the ...
Despite earlier claims, we could identify galactokinase activity in growing cells and all genes of the Leloir pathway (responsible for channelling d-galactose into the EMP pathway) are well induced on d-galactose (and also on lactose, d-xylose and l-arabinose) in the mycelial stage. Expression...
The addition of an aminoarabinose moiety to the lipid A core (a PbgPE-dependent LPS modification) decreases the initial electrostatic attraction by reducing the net negative charge on the outer membrane, thereby playing a role in bacterial virulence by increasing resistance to CAMPs24. In ...