However, when expression of the cloned misL gene was driven by the Escherichia coli arabinose promoter, MisL could be detected in the S. Typhimurium outer membrane by Western blot and on the bacterial cell surface by flow cytometry. Expression of MisL enabled S. Typhimurium to bind fibronectin ...
The nucleotide sugar UDP-glucuronic acid (UDP-GlcA) is the principal precursor for galacturonic acid, xylose, apiose and arabinose residues of the plant cell-wall polymers. UDP-GlcA can be synthesized by two different functional pathways in Arabidopsis involving either UDP-glucose dehydrogenase or ...
a previously uncharacterized putative trypsin-like serine protease, is required for male fertility. Prss37 is highly and exclusively expressed in the testis of adult mice, especially in the elongating spermatids during spermiogenesis, and almost vanishes in the mature sperm of mice. Mice deficient for...
In this strain, the native algD promoter has been replaced by araC-PBAD, allowing for high level inducible expression of the algD operon in the presence of arabinose. Clarified lysates were applied to agarose resin conjugated to anti-VSV-G mAbs, and the elution from the resin after washing...
pBAD24 Apr, expression plasmid with arabinose-inducible promoter [21] pKD46 Apr; expresses λ red recombinase [22] pKD3 cat gene, template plasmid [22] pGP tkt1 pGP704 derivative harboring tkt1 gene this study pBAD tkt1 pBAD24 derivative, tkt1 gene under the control of PBAD this study...
In this strain, the expression of FtsY is under the control of the arabinose promoter which allows the gradual depletion of FtsY by growing cells without arabinose. In comparison to wild-type INV, targeting of MtlA-189 RNCs to FtsY-depleted IY28 INV was significantly reduced (Figure 2A),...
Overexpression from genes under control of the arabinose promoter was achieved by addition of 0.2% arabinose to the medium. Cells were harvested at an absorbance at 600 nm of ∼1.0. Inverted membrane vesicles were prepared as described previously [7]. Vesicles were resuspended in 50 mM Tris-...
To complement the Δ PA4834 mutant, the arabinose-inducible expression vector pJN10563 was used. PCR was used to amplify the PA4834 gene along with the ribosome binding site (RBS) that originated from pET21b. The EcoRI/XbaI-digested PCR product was ligated into EcoRI/XbaI-digested pJN...
The addition of an aminoarabinose moiety to the lipid A core (a PbgPE-dependent LPS modification) decreases the initial electrostatic attraction by reducing the net negative charge on the outer membrane, thereby playing a role in bacterial virulence by increasing resistance to CAMPs24. In ...
Describe transcription control in the Arabinose (araBAD) operon. What does the process of post-translational control refer to? 1) Regulation of gene expression after transcription. 2) Regulation of gene expression after translation. 3) Control of epigenetic activation. 4) The period between ...