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Extreme instability of the ara activator ( ara C gene product) of Escherichia coli in a cell free protein synthesis system explains why a mutant activator that produces constitutivity of the arabinose operon in vivo does not do so in vitro ....
the present invention relates to the identification of a gene encoding a novel l-arabinose isomerase strain (bacillus stearothermophilus us100 have us100), l-arabinose isomerase expressed from said gene, the recombinant vectors for microorganisms hebergeant gene. trees with the recombinant vector.a pro...
which alters expression of a plasma membrane arabinogalactan protein4 epitope in the progenitors of the germ cells, and which subsequently affects the gametes, zygote and globular-stage embryo. 展开 关键词: arabinose Biotechnology development Gene expression grain legumes Immunology Peas sex Sexual ...
Arabinose is metabolized via a phosphoketolase pathway in Clostridium acetobutylicum ATCC 824doi:10.1007/s10295-012-1186-xIn this report, a novel zymogram assay and coupled phosphoketolase assay were employed to demonstrate that Clostridium acetobutylicum gene CAC1343 encodes a bi-functional xylulose-5...
a response regulator to regulate gene expression21. BYKs catalyze the phosphorylation of tyrosines via three Walker motifs, which are canonically involved in ATPase activities but here function as kinase domains22. The same active site, albeit only containing a Walker A and a Walker B motif, is...
The plasmid pKD46 (Datsenko and Wanner, 2000), which carries arabinose-inducible λRed genes, was used to provide the λRed recombination. To construct amplimers with an excisable KmR-marker and flanking sequences homologous to the dtd gene, we used the primers 5′-tgtttcccgtgacaaaccctgga...
By this way, as an instance, xylose is transformed into xylulose and then phosphorylated to xylulose 5-phosphate, arabinose into ribulose, and this in turn is phosphorylated to ribulose 5-phosphate (Gao et al., 2011; Mayo et al., 2010). Table 2. Microorganisms used in the production of ...
In this strain, the native algD promoter has been replaced by araC-PBAD, allowing for high level inducible expression of the algD operon in the presence of arabinose. Clarified lysates were applied to agarose resin conjugated to anti-VSV-G mAbs, and the elution from the resin after washing...
reaction antigene-anticorpsacacia senegalgomme arabiquearabinoseglycoproteine / galactanegoma arabigareaccion antigeno-anticuerpoglicoproteinas / galactanosarabinosaantigen antibody reactionsIsrael Journal of Botany 34:2-4, pages 113-128.doi:10.1080/00021369.1984.10866126Yukio Akiyama...