Focuses on the production of endopolygalacturonase (endoPG) by Colletotrichum lindemuthianum, a fungal pathogen causing anthracnose on bean seedling, which was enhanced when the fungus was grown in a liquid medium of L-arabinose or L-rhamnose. Molecular study of endoPG gene expression; ...
Like D‐xylose, L‐arabinose and D‐galactose were found to serve as substrates for NADPH‐linked reactions in extracts of cells grown in medium containing D‐xylose, L‐arabinose, or D‐galactose. These L‐arabinose and D‐galactose NADPH‐linked activities also appear to be inducible, since ...
We used this genetic background to select for pentose-derepressed mutants on media containing combinations of gluconate with xylitol, L-arabinose or D-xylose. A subset of these mutants was further analysed and turned out to have rather interesting properties as described in chapters two and three...
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E. coli were grown at 37 °C in LB, when necessary, with kanamycin (Km, 50 μg ml−1), ampicillin (Ap, 50 μg ml−1), l-arabinose (l-ara, 0.1% w/v) or d-glucose (glu, 0.2% w/v). M. smegmatis mc2155 was grown at 37 °C in LB, when necessary, ...
The enzyme also liberated L-arabinose from the β-L-arabinopyranosyl side chain of larch wood arabinogalactan. However, we could not detect any β-L-arabinopyranosidase activity or remarkable transcriptional induction in cultured cells of B. longum subsp. longum. Mutagenesis experiments revealed ...
The engineered strain BL7992 was cultured in shake flasks and the synthesis of melatonin was attempted by adding inducers (2 g/L arabinose and 1 mM isopropyl-β-D-thiogalactoside (IPTG)) and 1 g/L l-Trp as the substrate. The resulting supernatant was analyzed using UPLC–MS / MS to dete...
coli strain DH10B (Thermo Fisher Scientific) in LB media supplemented with 100 μg mL−1 ampicillin and 0.02% l-arabinose. Proteins were extracted using B-PER bacterial protein extraction reagent (Thermo Fisher Scientific) and tested for fluorescence brightness and lactate-dependent response....
This chapter describes the preparation of UDP-pentoses (UDP-D-xylose and UDP-L-arabinose). The UDP-pentoses can be prepared by two types of reaction catalyzed by plant enzymes. One of these types is catalyzed by UDP-D-glucuronic acid decarboxylase and the other by UDP-pentose pyrophosphorylas...
2b). Moreover, the enzyme exhibited much higher activity towards sugar beet arabinan than against the debranched sugar beet arabinan (releasing 480.7 and 64.4 mg/L arabinose, respectively), indicating that TlAbf51 preferentially acted on 1,2- or 1,3-linked arabinan residues debranched as side ...