Focuses on the production of endopolygalacturonase (endoPG) by Colletotrichum lindemuthianum, a fungal pathogen causing anthracnose on bean seedling, which was enhanced when the fungus was grown in a liquid medium of L-arabinose or L-rhamnose. Molecular study of endoPG gene expression; ...
We used this genetic background to select for pentose-derepressed mutants on media containing combinations of gluconate with xylitol, L-arabinose or D-xylose. A subset of these mutants was further analysed and turned out to have rather interesting properties as described in chapters two and three...
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coli strain DH10B (Thermo Fisher Scientific) in LB media supplemented with 100 μg mL−1 ampicillin and 0.02% l-arabinose. Proteins were extracted using B-PER bacterial protein extraction reagent (Thermo Fisher Scientific) and tested for fluorescence brightness and lactate-dependent response....
In order for the fungus microbe to utilize the L- arabinose, it can operate with gene manipulation. The L- arabinose route which is not known, namely, the gene of L- arabinitoru 4- dehydrogenase and the L- kishirurosu reductase was identified. These genes, the known gene of L- arabino...
The engineered strain BL7992 was cultured in shake flasks and the synthesis of melatonin was attempted by adding inducers (2 g/L arabinose and 1 mM isopropyl-β-D-thiogalactoside (IPTG)) and 1 g/L l-Trp as the substrate. The resulting supernatant was analyzed using UPLC–MS / MS to dete...
2b). Moreover, the enzyme exhibited much higher activity towards sugar beet arabinan than against the debranched sugar beet arabinan (releasing 480.7 and 64.4 mg/L arabinose, respectively), indicating that TlAbf51 preferentially acted on 1,2- or 1,3-linked arabinan residues debranched as side ...
In summary, we are showing for the first time an efficient biosynthesis ofd-xylitol froml-arabinose utilizing a filamentous ascomycete fungus. This broadens the potential resources to include also arabinan-rich agricultural waste streams like sugar beet pulp and could thus help to make alternative swe...
Industrial application of icariin and baohuoside I has been hindered by the short supply to a great extent. In this work, a novel GH78 α-l-rhamnosidase AmRha catalyzed the bioconversion of low-value epimedin C in crude Epimedium Folium flavonoids (EFs)
2.2. d-Xylose/l-arabinose and steam-exploded sugarcane induction A. niger spores were cultivated on complete medium (CM), composed by salt solution, trace elements and yeast extract, as described above for BCM, supplemented with 1% agar and the carbon source, at 30 °C for 3–5 days and...