铁离子比色法检测试剂盒(Intracellular Iron Colorimetric Assay Kit) E1042 描述:铁是人体必需的元素,对人体的新陈代谢和身体健康具有重要作用,血液中铁的 含量不足,就会造成缺铁性贫血。铁存在于人体所有的细胞内,各组织、器官包括各内分泌 腺都含有铁。肝脏、脾脏和肺组织内含量较为丰富。成人体内含铁 3...
北京普利莱基因技术有限公司 电话:010-62027915,62053186 Email: service_applygen@163.com 铁离子比色法检测试剂盒(Intracellular Iron Colorimetric Assay Kit) E1042 描述:铁是人体必需的元素之一,对人体的新陈代谢和身体健康具有重要作用,血液中铁的 含量不足,就会造成缺铁性贫血.铁存在于人体所有的细胞内,各组织...
Intracellular iron concentrations were determined by modified ferrozine-based colorimetric method (59). C. albicans WT exponential phase cells grown in 100 ml of LIM, RIM, or YPD were harvested, washed with iron-free water, and resuspended in 600 μl of 0.5 m NaOH. After addition of 300 mg...
isolation was performed according to instructions of M-PER®Mammalian Protein Extraction Kit (Thermo Scientific). After that protein concentrations of samples were determined with Pierce™ BCA Protein Assay (Thermo Scientific). Then, ICP-MS measurement was performed same as mentioned above. Determinat...
Here we develop a rapid colorimetric Pi imaging method, inorganic orthophosphate staining assay (IOSA), that can semi-quantitatively image intracellular Pi with high resolution. We used IOSA to reveal the alteration of cellular Pi distribution caused by Pi starvation or mutations that alter Pi ...
percentage induction over control. intracellular iron determination the intracellular iron ions content was determined in hearts and h9c2 cells using the colorimetric iron assay kit (abcam, spain), following the manufacturer's instructions. all assays were performed in duplicate, and measured on a ...
The colorimetric Alamar Blue assay measures the redox capacity of cells due to the production of metabolites, as a result of cell growth, and allows determination of viability over the culture period without the detachment of adherent cells. Cell viability was not affected significantly when DOTAP:...
Cells were lysed, and cAMP content was determined by a colorimetric competitive ELISA using a microtiter plate reader (Synergy HT). cAMP is expressed relative to protein content measured via BCA assay. B and C, HUVECs in culture on semiporous filters were treated for 60 min with DHA, 30 ...
We used a colorimetric assay based on the protocol of Tamaritet al.44to quantify the iron content of yeast cells and the purified protein. This method relies on the Fe2+-dependent optical absorbance of bathophenanthrolinedisulfonic acid (BPS) at 535 nm at pH 5.4. As standards, we dissolved...
Optimization of a multi-well colorimetric assay to determine haem species in Plasmodium falciparum in the presence of anti-malarials. Malar. J. 2015, 14, 253. [Google Scholar] [CrossRef] [Green Version] Combrinck, J.M.; Mabotha, T.E.; Ncokazi, K.K.; Ambele, M.A.; Taylor, D.;...