铁离子比色法检测试剂盒(Intracellular Iron Colorimetric Assay Kit) E1042 描述:铁是人体必需的元素,对人体的新陈代谢和身体健康具有重要作用,血液中铁的 含量不足,就会造成缺铁性贫血。铁存在于人体所有的细胞内,各组织、器官包括各内分泌 腺都含有铁。肝脏、脾脏和肺组织内含量较为丰富。成人体内含铁 3...
The intracellular iron ions content was determined in hearts and H9c2 cells using the colorimetric Iron Assay kit (Abcam, Spain), following the manufacturer’s instructions. All assays were performed in duplicate, and measured on a Tecan Infinite F200 microplate reader. Levels of total iron, as ...
To quantify the number of apoptotic cells further, flow cytometry using the FITC Annexin V Apoptosis Detection Kit I (BD Biosciences) was applied following the manufacturer's suggested protocol. Caspase-3 activity was measured using a Caspase-3 Colorimetric Assay kit (Abcam, Cambridge, UK) ...
Iron loading stoichiometries of the protein samples were computed by dividing the iron concentrations by the protein concentrations, as measured by the 660 nm Protein Assay (Thermo Scientific). High-throughput screening. We inoculated 1 Â 108 cells in a 20 ml SD-HIS medium containing 200 mg...
We used a colorimetric assay based on the protocol of Tamaritet al.44to quantify the iron content of yeast cells and the purified protein. This method relies on the Fe2+-dependent optical absorbance of bathophenanthrolinedisulfonic acid (BPS) at 535 nm at pH 5.4. As standards, we dissolved...
Intracellular iron concentrations were determined by modified ferrozine-based colorimetric method (59). C. albicans WT exponential phase cells grown in 100 ml of LIM, RIM, or YPD were harvested, washed with iron-free water, and resuspended in 600 μl of 0.5 m NaOH. After addition of 300 mg...
A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery Article Open access 16 February 2021 “Iron free” zinc oxide nanoparticles with ion-leaking properties disrupt intracellular ROS and iron homeostasis to induce ferroptosis Articl...