Human HMGB-1 (High Mobility Group Protein B1) ELISA Kit Sample Type: Serum, Plasma Sensitivity: 18.75 pg/mL Reactivity: Human Cell Ferrous Iron Colorimetric Assay Kit Sample Type: Cell Assay Time: 40 min Research Areas: Ferroptosis Angiotensin Converting Enzyme 2 (ACE2) Inhibitor Screening Kit ...
The intracellular MDA concentration was determined using a Cell Malondialdehyde Assay Kit (Colorimetric method, #A003-4-1, Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer’s recommended protocol. Cell ferrous ion (Fe2+) level analysis After removing the supernatant from ...
Iron assay Ferrous iron concentration was analyzed in U87MG, U251 and P3#GBM cells using an iron colorimetric assay kit (Iron Assay Kit, Abcam; Burlingame, California, USA), Detailed protocols are provided in Supplementary “Materials and Methods”. The iron concentration was calculated according ...
but not human pancreatic ductal epithelial cells or wild-type K-Ras PDAC cells.18This process can be blocked by the iron chelator deferoxamine, whereas it is enhanced by exogenous lysosomal form of iron.18In addition to iron chelator deferoxamine, the ROS inhibitors (e.g.,...
The intracellular level of ferrous iron (Fe2 +) was measured using an iron assay kit from Abcam. As lipid ROS and MDA are key end products of lipid peroxidation [29], we also assessed whether the c-Myc/miR-142-5p axis regulates lipid ROS generation and MDA production following erastin ...
Intracellular ferrous iron (Fe2+) levels were quantified with the iron assay kit (Abcam; ab83366) in accordance with the supplier’s protocol. Cells (density: 5 × 106 cells/10 cm plate) were subjected to B-exos or MB-exos treatment for 24 h before and after pretreatment with DMSO,...
Experimentally, the administration of PZH markedly increased the trivalent iron and ferrous ion, suppressed the expression levels of SLC7A11 and GPX4 proteins, and reduced the GSH/GSSG ratio in the liver tissues of DEN-induced HCC rats. Conclusions Our data offer an evidence that PZH may ...
TUNEL cell apoptosis detection kit was used to analyze cell apoptosis in brain tissues. Enzyme-linked immunosorbent assays and commercial kits were used to detect levels of inflammatory markers and oxidative stress markers. Ferrous Iron Colorimetric Assay Kit and Fe 2+ colorimetric assay kit were ...
the signal were acquired by confocal laser scanning microscopy (CLSM, Leica TCS SP8; Leica Microsystems, Wetzlar, Germany) and FCM. Cells were plated in 6-well at 2 × 105cells/well, an iron colorimetric assay kit (Abcam, Cambridge, UK) was used to detect intracellular ferrous iron; ...
The following protocol was used for the assembly of the ferritin nanocage: 1 mL of Denaturation Buffer (25 mM HEPES, 50 mM NaCl, pH 7.5) was added to the cell-free reaction after expression of ferritin and then heat-treated at 90 °C for 10 min. Ferrous sulfate heptahydrate...