Iron Assay Kit -Colorimetric-试剂 I291-50tests I291 50tests Liperfluo-细胞脂质过氧化物检测 L248-50μg*1 L248 50μg Liperfluo-细胞脂质过氧化物检测 L248-50μg*5 L248 50μg*5 Lactate Assay Kit-WST试剂盒 L256-200tests L256 200tests 乳酸检测试剂盒 Lactate Assay Kit-WST L256-50tests...
8448 IR Iron Assay 8468 GLY Glycerol Assay 8498 TG Triglyceride Assay 8518 TGA Total Glutathione Assay 8528 AA Ammonia Assay 8558 GSH/GSSG GSH/GSSG Ratio Assay 8588 Cho Choline Assay 8648 Mal Malate Assay 8268 MITOISO Mitochondria Isolation Kit 8278 COX Cytochrome C Oxidase Assay 8318 CS Citrate...
Cell Counting Kit-8是用于化学药物等对细胞增殖或毒性实验时进行细胞计数的试剂盒。试剂盒中使用高灵敏度的新型水溶性四唑盐WST-8甲臜染料作为显色底物,与传统的细胞计数试剂盒相比,灵敏度大幅度的提高。WST-8被细胞内的脱氢酶还原产生水溶性的甲臜染料。通过直接测量甲臜染料(450 nm)的吸光度,即可测出活细胞的数量...
100 ng of total RNA was used as input. First and second strands of cDNA were synthesized from total RNA (100 ng) spiked with 1 µl of 1:500 diluted ERCC ExFold RNA Spike-In Mix 2 (Life Technologies, Carlsbad, CA) at a final concentration of 1%. Following primer annealing and cDNA...
The intracellular iron concentration was quantified using a Total Iron Content Colorimetric Assay Kit (Applygen #E1042, Beijing, China) according to the manufacturer’s instructions. Transmission electron microscopy Cells cultured in a 10-cm plate were harvested with trypsin digestion, and fixed with ...
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity of CCK-8 is superior to that of other tetrazolium salts, including MTT, XTT, and MTS. Figure 1: Working mechanisms ...
a Total intracellular iron in U87MG, U251 and P3#GBM glioma cells after transfection with si-COPZ1#1 at 24 h. b Graphic representation of the levels of intracellular ferrous iron compared to ferric iron, as evaluated with the colorimetric iron assay kit. c Representative fluorescence images...
2.12. Intracellular iron measurement Using an iron colorimetric test kit (E-BC-K880-M, Elabscience, Wuhan), total intracellular irons were detected. In 6-well plates, LLC, H1299, and A549 cells were cultivated at a density of 2 × 105 cells per well. After receiving STD treatment for 24...
The iron colorimetric assay kit (E-BC-K880-M, Elabscience, Wuhan) was used to detect total intracellular irons. Briefly, H1299 and A549 cells were cultured in 6-well plates with a density of 2 × 105 cells per well. Following incubation with Tim-AIII for 48 h, cells were harvested an...
the signal were acquired by confocal laser scanning microscopy (CLSM, Leica TCS SP8; Leica Microsystems, Wetzlar, Germany) and FCM. Cells were plated in 6-well at 2 × 105cells/well, an iron colorimetric assay kit (Abcam, Cambridge, UK) was used to detect intracellular ferrous iron; ...