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Mice submitted or not to AMF exposure were perfused with 4% paraformaldehyde 1 day or 7 days post-treatment. After extraction of the brains, the tissues were frozen and subsequently cut in 12 μm-thick slices using a cryostat. Iron was detected using an iron stain kit (Sigma) in accordance...
Iron binding, naturally occurring protein bovine lactoferrin (bLf) has attracted attention as a safe anti-cancer agent capable of inducing apoptosis. Naturally, bLf exists partially saturated (15-20%) with Fe3+ however, it has been demonstrated that mani
Gel Retardation Assay Protein extracts were prepared from murine Ltk− cells that were treated for 16 hr with 100 μM desferoxamine mesylate (Sigma) as previously described (Pantopoulos and Hentze 1995). An α-32P-labeled ferritin H chain–IRE RNA probe was generated as described (Pantopoulo...
Sodium Iron EDTA 货号: YZ-1614239 品牌: 美国USP 暂无详情 产品简介 CAS15708-41-5 单位瓶 货期4-6周 规格200mg 血清铁浓度检测试剂盒 微量法,Micro Serum Iron Concentration Assay Kit,货号:BC1735- 100管/96样 发表于2022年6月27日由上海金畔生物科技有限公司 ...
Slides were then incubated in Perls’ stain (Iron assay kit, Sigma-Aldrich, Sweden) as per the manufacturer’s protocol for 30 minutes at room temperature (RT) and then washed with distilled water. For nuclear staining, slides were incubated with nuclear fast-red solution and incubated for ...