Iron assay Cardiac ferrous and total iron levels from frozen heart were determined using the Iron Assay kit (MAK025, Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol. Next generation sequencing (NGS)-based RNA-seq analysis C&K Genomics (Seoul, South Korea) perfor...
Iron has been shown to trigger oxidative stress by elevating reactive oxygen species (ROS) and to participate in different modes of cell death, such as ferroptosis, apoptosis and necroptosis. However, whether iron-elevated ROS is also linked to pyroptosi