The teams from Würzburg and Berlin tried to infect theintestinal organoidswith chlamydia. They discovered that the inner cell layer of the organoids is very resistant to the bacteria: the pathogens could only penetrate there if the cell epithelium was damaged. From the blood side, however, chla...
After preparation, store at 4 °C. B.2.5 Intestinal organoid culture medium: Advanced DMEM/F12 medium supplemented with factors including but not limited to N-2 Supplement, Serum-Free B27, GlutaMax, and other factors as needed for the experiment. B.2.6 Cell digestion solution: commonly used 0...
Organoid units (OU) are multicellular and contain both mesenchymal and epithelial cells, including the progenitor cells of the muscle, nerve, and epithelium of the intestine. Although intestinal epithelial stem cell culture of Lgr5 cells or of crypts is well described, no subs equent in vivo ...
Organoids derived from human duodenum and ileum were genetically modified using CRISPR-Cas9 to express the fluorescent protein Venus driven by the chromogranin-A promoter. Fluorescent cells from CHGA-Venus organoids were purified by flow cytometry and analysed by 10X single-cell RNA sequencing. ...
Representative models of intestinal diseases are transforming our knowledge of the molecular mechanisms of disease, facilitating effective drug screening and avenues for personalised medicine. Despite the emergence of 3D in vitro intestinal organoid cult
Using intestinal organoids, we show that transient p53 expression is required for the induction of revival stem cells and is controlled by an Mdm2-mediated negative feedback loop. Together, our findings reveal that p53 suppresses severe radiation-induced GI injury by promoting fetal-like ...
According to the latest related research, the introduction of carbon nanotubes in a 3D model within an intestinal organoid increased the number of Paneth cells, enteroendocrine cells, goblet cells and absorptive enterocytes forming mature crypt-villi lumen structures. On the one hand, carbon nanotubes...
Induced quiescence of Lgr5+ stem cells in intestinal organoids enables differentiation of hormone-producing enteroendocrine cells. Cell Stem Cell 20, 177–190.e4 (2017). CAS PubMed Google Scholar Alexandre, C., Baena-Lopez, A. & Vincent, J.-P. Patterning and growth control by membrane-...
OrgCult™-Intestine(XF)类器官生长培养基分别支持小鼠ASC和人ASC衍生的肠类器官的构建和长期维持培养。OrgCult™-Intestine(XF)肠类器官培养基可用于从人多能干细胞直接分化为肠类器官,通过3个阶段的分化,这些肠类器官可以在 OrgCult™-Intestine(XF)肠类器官生长培养基中进行维持培养和成熟。OrgCult™-Intes...
For cell culture, small intestine organoids derived from the intestinal stem cells were cultured alone or co‐cultured with macrophages (VDR+/+ or VDR/). After 3 days, the organoids and organoid‐macrophage co‐cultures were collected for western blots. Western blots were used to measure and ...