Intestinal organoids culture Small intestinal organoids were cultured according to a previously described protocol established by Sato and Clevers43. Crypts were collected by incubating the small intestine in PBS containing 2 mM EDTA, followed by isolation using a 70 mm cell strainer. Crypts wer...
The bridge between the understanding of intestinal regeneration and a therapeutic application of this knowledge toward the treatment of intestinal failure and short bowel syndrome lies in the ability to culture intestinal stem cells in three-dimensional units called organoids. Intestinal organoids are arr...
To model inflammation, organoids can be exposed to an inflammatory medium where standard growth medium is supplemented with different pro-inflammatory cytokines and bacterial components, or by introducing immune cells while maintaining standard organoid culture conditions Full size image The ability to ...
been de- and recellularized with myofibroblasts, endothelial cells, and epithelial cells (whole organoids or sorted cells) (Chen et al., 2016). This approach promises to provide additional insights into the normal gut physiology, not currently feasible with the canonical organoid culture protocol....
organoid culture, long-term studies of Paneth cells are now possible, rendering molecular and cell biological dissection of Paneth cell functions much more feasible5. Despite the numerous advantages, however, the intestinal organoid culture system comprised only of epithelial cells is short of recapit...
The intestinal epithelium isolated from chicken embryos in last 3 days of development can be used to establish the 3D culture of intestinal organoids. When fragments of epithelial tissue released by incubation with EGTA (2.5 mM, 2 h) are embedded in Matrigel matrix on cell culture inserts the ...
organoidsStudying the molecular basis of intestinal infections caused by enteric pathogens at the tissue level is challenging, because most human intestinal infection models have limitations, and results obtained from animals may not reflect the human situation. Infections with Salmonella enterica serovar ...
Erratum to: The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine.doi:10.1007/7651_2018_169MalgorzataDepartmentPierzchalskaDepartmentPanekDepartmentCzyrnekDepartmentMajaDepartmentGrabackaDepartmentMethods in molecular biology (Clifton, N.J.)...
Organoid units (OU) are multicellular and contain both mesenchymal and epithelial cells, including the progenitor cells of the muscle, nerve, and epithelium of the intestine. Although intestinal epithelial stem cell culture of Lgr5 cells or of crypts is well described, no subs equent in vivo ...
Prolonged in vitro culture of these cells for days to weeks has not led to better results when compared to implantation within hours of organoid unit isolation. However, other modifications in this protocol have proven to be successful in creating more robust intestinal neomucosa. For instance, ...