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4–1BB co-stimulation further enhances anti-PD-1-mediated reinvigoration of exhausted CD39+ CD8 T cells from primary and metastatic sites of epithelial ovarian cancers. J Immunother Cancer. 2020;8. Available from: https://doi.org/10.1136/jitc-2020-001650 Zheng GXY, Terry JM, Belgrader P, ...
We isolated CD45-negative cells from the single-cell suspension obtained from fresh pancreatic cancer tumor tissues using magnetic-activated cell sorting (MACS; Miltenyi Biotec). After thawing the cryopreserved single-cell suspensions, we depleted cell debris, dead cells, and dying cells using the De...