coli strain, a site-specific recombination event results in the excision of a plasmid replicon with the cloned insert. (3) Single-stranded DNAs can be recovered by growing helper M13 phages on bacteria harboring such plasmids. The vector, λMGU2, has been used to construct a nematode (...
Cloning of random oligonucleotides to create single-insert plasmid libraries - Worthington, Pelo, et al. - 2001 () Citation Context ...)5 oligonucleotide region containing randomized codons was converted to doublestranded DNA by primer extension with Klenow DNA polymerase prior to cleaving the ...
We successfully used this modified Gibson assembly protocol with two short insert-plasmid overlap regions, each counting only 15 nucleotides. 展开 关键词: PLASMIDS MOLECULAR cloning MOLECULAR self-assembly NUCLEOTIDE sequence SINGLE-stranded DNA
coli strain, a site-specific recombination event results in the excision of a plasmid replicon with the cloned insert. (3) Single-stranded DNAs can be recovered by growing helper M13 phages on bacteria harboring such plasmids. The vector, λMGU2, has been used to construct a nematode ( ...
such as plasmid-encoded receptor complexes and pili2-5. However, the tail-less, icosahedral, single-stranded (ss) DNA ΦX174-like coliphages do not fall into these well-defined infection paradigms. For these phages DNA delivery requires a DNA pilot protein. Here we show that the ΦX174 pi...
Cases have occurred with positive serologic testing (including positive anti-nuclear and/or anti-double-stranded DNA antibody testing). If EXTAVIA-treated patients develop new signs and symptoms characteristic of this syndrome, EXTAVIA therapy should be stopped. 5.12 Monitoring for Laboratory Abnormalities...
PNA probes were complexed with DNA targets in the following forms: single-stranded oligonucleotides, a loop of DNA in a hairpin conformation, and as supercoiled plasmid with the (G4T4G4)/(C4A4C4) insert. Gel-shift mobility assays ... TI Gaynutdinov,EA Englund,DH Appella,... - 《Nucleic...
DNA base-insertion enzymes (insertases) are a class of enzymes that catalyze the reinsertion of a missing base into the appropriate apurinic or apyrimidinic sites (AP sites) in DNA. The base-deoxyribose bonds in DNA are susceptible to spontaneous cleavages that release free bases and lead to...
(A) The one-step CRISPR approach uses a single site for gRNA directed, Cas9 mediated cleavage. A dsDNA donor plasmid is used for HDR and inserts 24xMS2 stem-loops into the endogenous gene locus. The donor plasmid also contains loxP sites and a marker gene. (B) For the two-step CRISPR...