Share on Facebook plasmid (redirected fromPlasmids) Thesaurus Medical Encyclopedia Related to Plasmids:Restriction enzymes plas·mid (plăz′mĭd) n. A circular, double-stranded unit of DNA that replicates within a cell independently of the chromosomal DNA. Plasmids are most often found in bacteri...
The present invention relates to novel recombinant plasmids capable of replicating in Escherichia coli characterized in that they contain a complete or partial sequence of the double or single-stranded DNA of a densovirus causing densovirus infection in a susceptible insect. The invention also relates...
One strand of the double stranded plasmid is cut, then transmitted from the donor to the recipient through the pilus. Complementary strands for the single strands remaining in the donor and recipient are created, hence both bacteria now have a copy of the original plasmid. The pilus is removed...
Site-directed mutagenesis of double-stranded plasmids, domain substitution, and marker rescue by comutagenesis of restriction enzyme sites. 来自 NCBI 喜欢 0 阅读量: 27 作者:JA Nickoloff,WP Deng,EM Miller,FA Ray 摘要: Oligonucleotide-directed mutagenesis can be used to introduce specific alterations ...
In the first step, synthesis of the complementary strand converts the single-stranded viral DNA into a double-stranded replicative form (RF). Viral or (+)-strand DNA replication in the second step is initiated by the introduction of a specific nick on the (+) strand of the RF molecule ...
(ssDNA) into double-stranded DNA (dsDNA)20. The regulation of some of these genes involves unique promoters designated Frpo, which adopt a secondary structure that mimics a double-strand conformation, allowing recognition by the host RNA polymerase6,17. Thus, Frpofunctions as a single-strand ...
Single-stranded (ss) DNA viruses are a major component of the earth virome. In particular, the circular, Rep-encoding ssDNA (CRESS-DNA) viruses show high diversity and abundance in various habitats. By combining sequence similarity network and phylogenet
Although thought initially to regulate transcription of the rom gene, it was shown subsequently to increase the rate of binding of the RNA I repressor to RNA II (Tomizawa and Som, 1984) by interacting with the double stranded stems of inhibitor and target (Helmer-Citterich et al., 1988). ...
Plasmids are extrachromosomal genetic materials that are generally referred to as autonomously replicating double-stranded, circular or linear DNA molecules44. About 25% of red algal genera contain more than two plas- mids per species, and encode open reading frames (ORFs) that are transcriptionally ...
Furthermore, due to lack of any extraneous sequence, synthetic linear double stranded DNA contains exclusively the therapeutically relevant functional gene of interest, Dhir says. “This attribute offers wide benefits in both packaging of DNA in AAV vectors or non-viral vectors, with feasibility to...