Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process.Grimsley, Nigel Harry...
DNA sequence of interest was trimmed at the N-termini and C-termini before being subcloned into a staging vector. In vitro transposition reactions were set up following an established protocol71with slight customizations: 150 ng of the staging plasmid and 150 ng transposon were mixed with ...
As described herein, the term “replicon” means a replication unit capable of having voluntary control, and the replication unit is a continuous DNA molecule in which the replication is initiated at specific site within a molecule and terminated after sequential progress. All of the plasmid, viral...
RF cloning: A restriction-free method for inserting target genes into plasmids Restriction-free (RF) cloning provides a simple, universal method to precisely insert a DNA fragment into any desired location within a circular plasmid, i... FVD Ent,J L?We - 《J Biochem Biophys Methods》 被引量...
Unusual DNA structuresHere, we describe a methodology that allows the insertion of site-specific DNA lesions into genomes in living cells. The technique involves the integration of a plasmid containing a site-specific lesion engineered in vitro into a precise location in the genome via the site-...
This invention provides A method for preparing mutant genes, which comprises the steps of constructing a recombinant plasmid DNA by inserting a gene fragment into a plasmid DNA having a unidirectional origin, introducing the recombinant plasmid DNA into host cell lacking DNA error-correcting function ...
Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process.BARBARA HOHN...
Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process.NIGEL HARRY GRIMSLEY...
Novel transposable cloning vector and method of using it for the insertion into the chromosome of a recipient Gram-negative bacterium of DNA material foreign to that bacterium. The vector comprises first and second plasmids. The first plasmid carries a transposon (A), a part of whose DNA ...
production of hybrid dna corresponding to hepatitis a virus rna by inserting double strand dna into a bacterial plasmid; use for vaccine productiondoi:10.1016/0264-410X(86)90150-7ELSEVIERVaccine