Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperature and a much higher or totally uncontrolled copy number at a different temperature. The plasmid may be ...
coli reveals filament bundles involved in plasmid DNA segregation. Science 323, 509–512 (2009). Article CAS PubMed Google Scholar Studer, D., Klein, A., Iacovache, I., Gnaegi, H. & Zuber, B. A new tool based on two micromanipulators facilitates the handling of ultrathin cryosection...
These vectors also contain the Vir-region, necessary for the transfer of the T-DNA. Intermediary vectors cannot be replicated in Agrobacteria. However, by using a helper plasmid, the intermediary vector can be transferred to Agrobacterium tumefaciens (conjugation). Binary vectors can be replicated ...
Preparing L-threonine using bacteria of the Enterobacteriaceae family which produces L-threonine by inoculating and culturing the bacterium in a first nutrient medium, and continuously supplying the n
DNA SEQUENCE ENCODING A SPECIFIC L-ARABINOSE TRANSPORTER, A cDNA MOLECULE, A PLASMID COMPRISING THE SAID DNA SEQUENCE, HOST CELL TRANSFORMED WITH SUCH PLASMID AND APPLICATION THEREOF The invention is useful for the complete use of sugar obtained from plant biomass and other ligno-cellulose materials...
used to transformB. subtilis. Finally, objective DNA was obtained as plasmid DNA. Full size image To demonstrate the merits that come from the strict standardization of OGAB block size, we performed two types of DNA assemblies: reconstruction of the lambda phage genome and synthesis of ade ...
The techniques for preparing such multi-plasmid strains from bacteria of the genus Pseudomonas are described. Living cultures of two strains of Pseudomonas (P. aeruginosa [NRRL B-5472] and P. putida [NRRL B-5473]) have been ... AM Chakrabarty - 《Biotechnology》 被引量: 68发表: 1992年 ...
Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperature and a much higher or totally uncontrolled copy number at a different temperature. The plasmid may be ...
Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperature and a much higher or totally uncontrolled copy number at a different temperature. The plasmid may be ...
A main mechanism of lateral gene transfer in bacteria is transformation, where cells take up free DNA from the environment which subsequently can be recomb... M Vos,A Buckling,B Kuijper - 《Trends in Microbiology》 被引量: 0发表: 2019年 Glycine cleavage enzyme complex: molecular cloning and ...