In vitro GST pull-down The plasmids (Myc-PTEN or Myc-DUSP7) were transiently transfected into 293T cells. After transfection for 24 h, the cells were harvested and lysed in cell lysis buffer for Western and IP (Beyotime, P0013) containing protease inhibitor on ice. GST or GST-SPOPMATH...
GST pull-down assay demonstrating in vitro binding between MT-1X and FHL3.doi:10.1371/journal.pone.0093723.g003C. XinW. JinfengH. XinFu WenliangX. WenrongZou MinjiWang YuanyuanWang JiaxiXu Donggang
We performed an in vitro pull-down assay to validate the interaction between OsUBC12 and OsSnRK1.1. OsSnRK1.1-His, OsUBC12-GST and GST alone were all detected in whole-cell lysates (Input). OsSnRK1.1-His was pulled down by OsUBC12-GST, but not by the GST-only control, suggesting th...
Another report demonstrated that the intact ERα F-domain inhibited the association of unliganded ER with REA (Repressor of Estrogen Receptor) using in vitro GST pull down assays [40]. These studies indicate that the F-domain of ERα was responsible for the inhibition of ERα association with...
This assay thus allows to reconstitute the basic elements of more complex in vivo situations, where several objects pull on several MTs, for example, when organelles pull on front versus back MTs of a centering aster [6]. Download: Download high-res image (617KB) Download: Download full-...
To this end, we used a pull-down assay with the GST-RAF1-Ras binding domain (RBD), which specifically binds the GTP-loaded active form of Ras. 8505c and TPC-1 cells were transiently transfected with empty vector (pFLAG) or PD-1 (pFLAG PD-1) in combination with pCEFL H-Ras AU5 ...
p38 delta/MAPK13, Human, 重组蛋白 (GST), Activated in vitro,Product IntroductionBioactivity英文名: p38 delta/MAPK13 Protein, Human, Recombinant (GST), Activated in vitro描述: The p38 family of mitogen-activated protein kinases (MAPK) includes p38 alpha (
When cells are cultured in vitro, they live and grow in conditions very different from the physiologic environment. Therefore, facing the need to adapt, cells use metabolic routes that allow them to survive and even thrive in the new media. Such adaptations result from epigenetic events [168]....
No significant difference was detected in the amount of IKK1 and IKK2 protein in the respective BMDC lysates (Figure 2B), indicating that the lack of in vitro phosphorylation of the IκBα-GST substrate was due to reduced IKK activity and not inefficient immunoprecipitation of the IKK complex....
An in vitro GST pull-down assay showed that purified GST-Twa1 directly interacted with His-β-catenin (Supplementary information, Figure S7A). Immunoprecipitation analysis revealed that either endogenous or ectopically expressed Twa1 interacted with endogenous β-catenin (Supplementary information, Figure...