awill actually be prescribed macrolides 意志实际上是被规定的大环内酯[translate] a技术部门仍在处理中 Technical department still in processing[translate] aIn vitro pull-down assay of the recombinant SlCIPK2 showed that 再组合SlCIPK2的体外拉下来分析用试样显示了那[translate]...
aIn vitro pull-down assay of the recombinant SlCIPK2 showed that SlCIPK2 interacts with SlCBLs and stress-responsive transcription factors, SlERF7, SlCBF1 and SlAREB1. 再组合SlCIPK2的体外拉下来分析用试样表示, SlCIPK2与SlCBLs和注重敏感副本因素, SlERF7、SlCBF1和SlAREB1互动。[translate]...
In Vitro HisTag Pull-Down Assay Using MagZ™ ParticlesBecky GodatTonny Johnson
In vitro pull-down assays Five microgram of pSG5-FLAG GFP (generously donated by Dr Samuel Buchsbaum) or 5 μg of pcDNA-FLAG-SMG5 and 5 μg of pcDNA-FLAG-SMG7 or 5 μg of pcDNA-FLAG-SMG6 and 10 nM UPF1 siRNA were transfected in 10 cm dishes using jetPRIME (Polyplus...
The presence of an endogenous interaction and the results of the in vitro pull-down assays revealed that DC-SIGN binds directly with TLR4. We also present evidence that DC-SIGN mediates TLR4-regulated NFκB activation but not activation of p38 and JNK. Our results suggest an essential role of...
2.In Vitro Enzyme Assay定义 通过将目的基因转入载体,后导入工程菌,诱导其表达获得有功能活性的蛋白,并构建含有该蛋白和底物的反应体系进行反应,以验证蛋白是否具有目标代谢物的催化作用。 3.In Vitro Enzyme Assay项目流程 In Vitro Enzyme Assay项目流程包括项目售前评估,基因克隆,载体构建,原核蛋白表达,蛋白纯化,与...
2. In Vitro Enzyme Assay定义 通过将目的基因转入载体,后导入工程菌,诱导其表达获得有功能活性的蛋白,并构建含有该蛋白和底物的反应体系进行反应,以验证蛋白是否具有目标代谢物的催化作用。 3. In Vitro Enzyme Assay项目流程 In Vitro Enzyme Assay项目流程包括项目售前评估,基因克隆,载体构建,原核蛋白表达,蛋白纯化...
2. In Vitro Enzyme Assay定义 通过将目的基因转入载体,后导入工程菌,诱导其表达获得有功能活性的蛋白,并构建含有该蛋白和底物的反应体系进行反应,以验证蛋白是否具有目标代谢物的催化作用。 3. In Vitro Enzyme Assay项目流程 In Vitro Enzyme Assay项目流程包括项目售前评估,基因克隆,载体构建,原核蛋白表达,蛋白纯化...
放射assayvitro受体抗原分析 ZhangYongxue Partone: Parttwo: Partthree: 体外放射分析 非放射标记免疫分析放射免疫分析免疫放射分析受体放射分析放射受体分析化学发光免疫分析时间分辨荧光免疫分析酶免疫分析荧光免疫分析等 1960年美国的Berson和Yalow将核技术与免疫学技术相结合建立了放射免疫分析法,并首先用于测定血浆胰岛素...
To explore the function of OsSRF3 in the PTI response, we performed a Y2H assay and identified 16 OsSRF3-interacting proteins (Supplementary Table 2). Notably, the brassinosteroid receptor kinase OsBAK1 was one of the interactors of OsSRF3. Therefore, we performed in vitro pull-down assays ...