The principle of pull-down assay is affinity purification, which is similar to immunoprecipitation, except that this method uses bait protein, instead of antibody to trap the target protein. In a pull-down assay, a bait protein is tagged and immobilized on beads with affinity ligand specific for...
gst相互作用蛋白质蛋白pulldown技术 ·综述·GST-pulldown技术在蛋白质相互作用中的应用柴政斌1,2,张更林1,韩金祥11.卫生部生物技术药物重点实验室山东省罕少见病重点实验室山东省医药生物技术研究中心山东省医学科学院,山东济南250062;2.济南大学山东省医学科学院医学与生命科学学院,山东济南250062摘要:蛋白间相互作用在...
A beads to IP, the A pull down from the cell, and specific antibody (such as phosphorylation, ubiquitination of antibody) to detect changes in A. The co-Ip principle is the same, since it is detected and the interaction of A protein, that is using the A antibody to pull A down, ...
This is termed a ‘GST pull-down’ assay, and many variants of it exist (7). GTPases-RAS and Ras-Related Ras and Ras-related, small molecular weight GTPases function in the regulation of signaling and cell growth control and collectively serve to control cell proliferation, differentiation ...