8. We recommend use of 5μl of the immobilized GST-fusion or GST control for the Phase 3 pull-down assay (Section 4.C). Thus, 20μl of particles will provide sufficient material for more than one pull-down assay. However, in some cases more than 5μl may be required for one pull...
Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.Features of GST Tag Protein Interaction Pull-Down Kit:• GST pull-down (Product No. 21516)—gently purifies ...
Our GST Pull-Down Assay service process mainly includes Experiment design based on your specific project needs (buffers, beads, tag of bait, etc); Parameters optimization, such as binding time; Cell lysis, pull-down assay(binding, washing, and elution); WesternBlot/ mass spectrometry ...
Pull-DownLysisBuffer,250mLGlutathione,1gBupH TrisBufferedSaline,1pack,25mMTris•HCl,0.15MNaCl,pH7.2whenreconstitutedwith500mLofultrapurewaterPierceSpinColumnsAccessoryPack,27columnswithpre-insertedfritandtopandbottomcapsCollectionTubesandCapsAccessoryPack,100graduated2mLtubesandplugcapsStorage:UponreceiptstorePull-...
The fusion protein can be stored on the beads at 4°C at this stage. This is appropriate if the protein is to be labeled or used in a GST pull-down experiment. Add 5 ml of ice-cold PBS supplemented with protease inhibitors. Resuspend the beads with gentle mixing. ...
Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.Features of GST Tag Protein Interaction Pull-Down Kit:• GST pull-down (Product No. 21516)—gently purifies ...
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center. There are Pull-Down Kits and Far-Western Kits. The Pull-Down Kits are very similar to an immunoprecipitation (IP), but instead of an antibody, a tagged bait protein [biotin (Cat. ...
Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.Features of GST Tag Protein Interaction Pull-Down Kit:• GST pull-down (Product No. 21516)—gently purifies ...
('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, ...