In vitro testing technologies for inhalation toxicology & skin exposure testing. Turnkey solutions for in vitro research at the highest technology level.
Using the in vitro degradation assay mentioned above we could demonstrate that the PELB stability was improved 500–1000 times in culture supernatants of some single prt mutants. This dramatic increase in the in vitro stability of the PELB tester protein, resulting from just one single mutation, ...
cell migration and invasion. The transwell migration assay involves the chemotaxis of cells through a porous membrane after the establishment of a chemoattractant gradient using two medium-filled compartments. The transwell invasion assay involves the addition of an extracellular matrix on top of the ...
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-sc... W Voigt - 《Methods in Molecular Medicine》 被引量: 241发表: 2005年 Efficacy of cytotoxic agents used in the treatment of testicular ge...
In-VitroCell 直热式CO2培养箱内置有145℃高温干热灭菌程序,和95℃高温湿热灭菌程序,可彻底消除培养室内潜在污染。NuTouch屏幕上可显示提示,一步步指导用户进行消毒程序。箱内组件如搁板,在灭菌过程中不需要取出。闭合环状HEPA过滤系统 结构 In-VitroCell CO2培养箱的设计特点和ISO 5级洁净室相似。培养室...
2.3 In vitro cell viability assay The in vitro cell viability assay was performed using the trypan blue exclusion test and MTT test. The effects of SA, HAESA and free HA samples were determined on cell viability of normal human osteoblast cell line (NHOst) that was purchased from Lonza. N...
In contrast, the degradation of the proteins used in this work can be ignored as no degradation tags were associated with them. However, while realizing the integral controller in vivo, the adverse effect of degradation on reference tracking due to cell dilution should be considered21. To test ...
For cell proliferation assay, T-cells were labeled with 1 μM CFSE (eBioscience, San Diego, CA) for 6 min and then cultured in the presence of 0.1 mg/ml plate-bound OKT3 (eBioscience, San Diego, CA). Cells fluorescence was analyzed by flow cytometry. Cell viability assays were...
This study explored the potential of cell-free supernatants (CFS) from 2 probiotic Lactobacillus sp., Lactobacillus casei and Lactobacillus rhamnosus GG, to inhibit colon cancer cell invasion by influencing matrix metalloproteinase-9 (MMP-9) activity and levels of the tight junction protein zona ...
mm2after 7 days). The CCK-8 assay was used to determine cell viability, and the results were shown in Fig.4d, e. Most impressively, compared with the rest of the samples, the (rhCol III/PDA-PEI)2sample showed the best cell viability at the designated time points; however, the cell ...