In vitro mucin degradation assay.Sirintra NakjangDidier A. NdehAnil WipatDavid N. BolamRobert P. Hirt
SusanaProençaBeate I.EscherFabian C.FischerCiaránFisherSébastienGrégoireNicky J.HewittBeateNicolAliciaPainiNynke I.Kramer, inToxicology in Vitro, 2021 2.6Abiotic degradation Abiotic degradation of test chemicals may occur inin vitroassays, thus decreasing Cmediumand Cfree. Some chemicals, such as...
We demonstrated that AZ’6421 is a potent degrader of ERα in vitro in multiple cell lines. The loss of ERα was rapid, with a half-life of 0.5 hours, and dependent on the proteasome, confirming that the reduction in ERα levels was due to degradation and not transcriptionally mediat...
High-throughput assays will also allow for conduction of more realistic in vitro drug testing assays, where many different compounds can be tested in parallel. These will hopefully provide a rational basis for the design of clinical studies that may ultimately open new ways to tackle bone ...
The in vitro pharmacology team have expertise across a variety of therapeutic areas, target types, assay formats and instrument, can offer services from target validation to screening compound with all kinds of biochemistry assays, biophysical assays, and cellular assays. ...
Cuproptosis, caused by excessively high copper concentrations, is urgently exploited as a potential cancer therapeutic. However, the mechanisms underlying the initiation, propagation, and ultimate execution of cuproptosis in tumors remain unknown. Here,
(89%), we hypothesized that Vpr also interacts with hHR23B and investigated this possibility using in vitro and in vivo assays. Indeed, GST pull-down experiments indicated an interaction between Vpr and hHR23B, with the binding region localized to the C-terminal half of hHR23B (Fig.4a). ...
The nuclear factor erythroid 2-related factor 2 (Nrf2) is a key regulator of cellular defense against oxidative stress and correlated with classical toxicological endpoints. In vitro methods using fish cell lines for the assessment of aquatic toxicity ar
In vitrodigestion assays simulate the physiological conditions of digestionin vivoand are useful tools for studying and understanding changes, interactions, as well as the bioaccessibility of nutrients, drugs and non-nutritive compounds. The technique is widely used in fields such asnutrition, pharmacolog...
Degradation and sensitivity: Over time and under real-world conditions, there could be degradation of materials or loss of sensitivity, which are crucial for the practical implementation of biosensors. Cost and accessibility - Synthesis and integration costs: The cost associated with the synthesis and...