doi:10.1016/0161-5890(79)90085-3D.G. RomansC.A. TilleyK.J. DorringtonElsevier LtdMolecular ImmunologyRomans DG, Tilley CA, Dorrington KJ. Interactions between Fab and Fc regions in liganded immunoglobulin G. Mol Immunol. 1979 Nov; 16 (11):859–879....
HBAT is composed of <2% intact immunoglobulin G (IgG) and [greater than or equal to] 90% Fab and F[(ab').sub.2] immunoglobulin fragments; these fragments are created by the enzymatic cleavage and removal of Fc immunoglobulin components in a process sometimes referred to as despeciation. ...
(D) While the hinge region holds the central area of the Ig molecule rigid, each Fab arm is able to rotate around the VH−CH1 axis. Flexibility in the secondary structure outside the hinge region also allows both the Fab and Fc portions of the molecule to move back and forth, to ...
Two methods of obtaining Fab and Fc fragments from mouse immunoglobulin Gl are described. In the first case the papain protein digest is fractionated on a column with DEAE-or DE-32-cellulose, equilibrated with 0.005 M potassium phosphate buffer, pH 8.0. The Fab fragment is eluted from the col...
The salivary lysozyme and immune globulin concentration are regarded as a sensitive sign which reflects a first- stage of danger of air pollution. 本文对采暖期两种不同采暖方式进行了一氧化碳(CO)污染对小学生碳氧血红蛋白(COHb)和免疫水平影响的比较研究,结果表明:co污染对小学生机体非特异性免疫和特异...
一个Fab分子能与一个抗原分子结合,Fc片段易于形成结晶,它与抗体的几种免疫功能有关。两个Fab片段和一个 Fc片段之间有一个铰链区,铰链区含有相当多的脯氨酸,且重链间的二硫键也多集中在这一区域,因此铰链区的肽链松散,容易被蛋白水解酶水解,有淋巴滤器之称。主要表现于单核吞噬细胞的吞噬作用(属于非特异性免疫)...
木瓜蛋白酶--Fab2+Fc1（有抗原性）胃蛋白酶—Fab2+pFc（无功能）二、Ig的生物学功能 1.抗体特异性结合抗原(bondingantigen)特点:(1)抗原抗体构型互补:抗体可变区中的高变区(HVR123)与抗原表位结合，抗原嵌入抗体CDR形成的口袋中，两种不同抗原带有共同抗原表位时可与同一种抗体结合造成交叉反应。(2)...
Fab fragments are obtained under both temperature conditions, and an Fab dimer has been identified in high temperature digests. This unusual difference in the products of enzyme digestion with temperature is unexplained but may be related to steric changes induced in the IgM molecule by heat. Fc ...
There are various severe health conditions that can lead to low levels of Immunoglobulin A in the body, one of which is gonorrhea. The bacteria that cause gonorrhea produce an enzyme that splices IgA antibodies into Fc and Fab fragments. The Fab part...
In subject area: Pharmacology, Toxicology and Pharmaceutical Science An IgG antibody is composed of two antigen-binding Fab domains that are linked by a flexible hinge region to a glycosylated Fc domain that mediates immunological functions (Benucci, Saviola, Manfredi, Sarzi-Puttini, & Atz...