W. Yi, D. Yang, Z. Xu, Z. Chen, G. Xiao, L. Qin Immortalization of mouse primary astrocytes Gene, 865 (2023), Article 147327 View PDF View article Cited by (0) Recommended articles cannot be displayed at this time. Captures Readers10 View details...
Garcia-Mesa Y, Jay TR, Checkley MA, Luttge B, Dobrowolski C, Valadkhan S, Landreth GE, Karn J, Alvarez-Carbonell D (2017) Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system. J Neuro-Oncol 23:47-66...
Immortalized cells can be isolated directly from an H-2Kb-tsA58 mouse simply by dissociation of the tissue of interest. First, they are cultured at 33 °C to obtain the required number of cells and then at 37 °C to provide the differentiation into various phenotypes as described previously...