Protein molecules are composed of light elements (mainly C,O,N and H) which give very little contrast and are so sensitive to radiation damage in the electron microscope that it is impossible to observe many details in a protein molecule before it has been destroyed by the electron beam. ...
A microscopic image recognition system for detecting a protein-based molecule by presenting a recognition image is provided. The protein-based molecule has a state of a monomer. The microscopic image recognition system includes an image capturing unit, a monomer tracking module and a texture mask. ...
Protein molecules are composed of light elements (mainly C,O,N and H) which give very little contrast and are so sensitive to radiation damage in the electron microscope that it is impossible to observe many details in a protein molecule before it has been destroyed by the electron beam. ...
Paraformaldehyde is small molecule that penetrates cells/tissue quickly, fixing proteins and other molecules in place. Glutaraldehyde is a bigger molecule with two reactive aldehyde groups that increase the potential for fixation and help create long chains of glutaraldehyde, generating a matrix. This ...
Professor von Itzstein said the 3-D image shows the architecture and intimate atomic-level detail of a bacterial heparanase, anenzymethat degrades a sugar molecule known asheparan sulfate. He said the functionally identical human enzyme is over-expressed in cancers and is known to be associated ...
-represented in specific assays, for example, 'protein localized in cytosol phenotype' (CMPO_0000393). Nonetheless, several phenotypes were observed in multiple orthogonal assays (e.g., 'round cell' (CMPO_0000118) and 'increased nuclear size' (CMPO_0000140)).Figure 1summarizes the sampling of...
Using mirror-image trypsin digestion and LC–MS/MS, we sequence a mirror-image large subunit ribosomal protein (L25) and a mirror-image Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4), and distinguish between different mutants of D-Dpo4. We also perform writing and reading of digital ...
Although single-molecule sensitivity has been achieved with these far-field techniques (Nie et al., 1994; Schwille et al., 1999), their spatial resolution is classically limited by diffraction, and temporal resolution is greatly sacrificed due to the scanning. A number of recent techniques have ...
Background: Spermatozoa are able to sense an attractant molecule gradient and as a consequence, orient their movement towards the source of the attractant.This mechanism is known as sperm chemotaxis. In recent years, our laboratory contr... LC Giojalas,HA Guidobaldi,ME Teves,... 被引量: 0发...
the spatial organizationof cellular structures and components. The assay is often used during drug screening to profile small molecules or other compounds on stained proteins in living cells. The pattern of cellular staining may revel morphological differences in samples treated by small molecule ...