Mos is also a component of cytostatic factor, an activity that is believed to arrest oocyte maturation at meiotic metaphase II. There is evidence showing that the Mos protein is associated with tubulin in unfertilized eggs and transformed cells, raising the possibility that it is involved in the...
A prometaphase muntjac skin fibroblast stained with Alexa Fluor® 350 phalloidin, an anti–a-tubulin antibody and an anti–cdc6 peptide antibody. Go › Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX ...
The next example targets cytogenetic images of human metaphase cells. This is a classical application of area opening. The task is to preprocess the image by segmenting out the chromosomes from the nuclei, stain debris, and the background. Fig. 6.8 shows the input image (a), the thresholded...
Interestingly, the images show that while RCPs are clearly overlapping with the nuclear stain during interphase, the RCPs are enriched around the metaphase plate in cells in mitosis, when the nuclear membrane collapses ahead of cell division, which has previously also been demonstrated for the ...
The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spi...
//chromosome.med.niigata-u.ac.jp/chronis/servlet/chronisservlet. The system enables users to input new images into the database, to locate images of interest by keyword searches, and to display the images with detailed information. CHRONIS has a wide range of applications, such as searching...
Cytogenetic analysis of Polyodon spathula匙吻鲟的细胞遗传学分析 Metaphase chromosome specimens of Polyodon spathula, injected with PHA and colchicin, were prepared from short-term culture of kidney cells with air-drying... XUE ShuQun,YIN HongBin,薛淑群,... - 《水生生物学报》 被引量: 0发表: ...
(e) Gates for anaphase/telophase and late prophase/metaphase (data is plotted for all human HT29 cells in the experiment [7]. X-axis = integrated nuclear intensity of DNA, log scale; Y-axis = mean nuclear intensity of phospho-histone H3). Random cells falling within the gates are shown...
The cell cycle stage prediction is represented as probability distributions over 9 classes, namely: “Early G1”, “Late G1”, “S/G2”, “Metaphase”, “Anaphase”, “Telophase”, “Abberant”, “Over_seg”, and “Anaphase_defect”. Meanwhile, the protein localization is represented as ...
MINS accurately detects nuclei with condensed chromosomes or dividing nuclei during metaphase (Figure S1, yellow arrowhead). However, MINS does not distinguish these nuclei from interphase nuclei during the detection process; to this end, an increased signal intensity of these nuclei correctly reflects ...