a. Picture of a metaphase II mouse oocyte with the picture of a micrometre grid (10-μm intervals) superimposed. b. Zygotes were filmed during injection with a gentle flow of concentrated suspension (0.2 mg/mL), but only up to the filling of the perivitelline space with an injected ...
In our case, the first biopsy suggested PMF inconsistent with the clinical findings while the second biopsy revealed a picture of AIMF. The patient had a negative autoimmune panel suggesting primary AIMF. The patient developed mucormycosis that first presented with nonspecific symptoms, although rare...
Our results highlight the importance of considering the imaging conditions used during model development and training. As an explorative study, we aimed to comprehensively include imaging conditions to provide a complete picture of the model’s performance across various imaging settings. In practice, ...
Giemsa banding Banding pattern observed in spreads of metaphase chromosomes, treated briefly with protease then stained with Giemsa. Can be used to identify the separate chromosomes. The deeply-staining G bands do not coincide with the pattern of quinacrine bands (Q bands). gbb The Drosophila ortho...
A prominent example of active large-scale flows is the poleward movement of microtubules observed in metaphase spindles11,12. Spindles are self-organized molecular machines responsible for the segregation of sister chromatids during cell division. They are made of dynamic microtubules that are continuou...
6. Studies have revealed that Mph1 (Ttk homologue), which localizes to the kineto- chores only at prometaphase (second phase of mitosis), is required for the recruitment of Bub1 and other spin- dle assembly checkpoint components [105, 106]. Ttk promotes closed Mad2l1 production and ...
The cells were subsequently arrested in metaphase by the addition of colcemid to a final concentration of 0.1 µg/ml (Life Technologies, Inc.). Harvested cells were incubated in 0.075 m KCl for 25 min at 37 °C and fixed in methanol/acetic acid (3:1) solution, and chromosome spreads ...
bind and stabilize MTs in axons8,9, possibly playing a role in the development of axonal branches10. It has been implicated in the growth of metaphase spindles in neural stem cells11and its upregulation has been observed in different forms of cancer12,13,14. Additionally, MAP7 is a ...
dividing without centromeres and metaphase plates) during vegetative growth and only differentiate from zygotic nuclei following conjugation (e.g. Gao et al. 2016; Raikov 1982). Throughout the remainder of this manuscript, we use the abbreviation Div− to describe the absence of macronuclear ...
Region of the mitotic spindle where chromosomes are aligned at metaphase: as its name suggests, it lies midway between the poles of the spindle. equilibrative nucleoside transporters Integral membrane proteins with 11 transmembrane domains that transport nucleosides in a sodium-independent fashion. In hu...