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Automatic metaphase analysis of Chinese hamster cells with a chromosome image analyzing systemMOUSE SPLENOCYTE ASSAYBIOLOGICAL MONITORINGX-RAYSFISSION NEUTRONSBLEOMYCINA modified mouse splenocyte culture system was standardized after testing different mitogens (i.e., phytohemagglutinin (PHA), concanavalin ...
The next example targets cytogenetic images of human metaphase cells. This is a classical application of area opening. The task is to preprocess the image by segmenting out the chromosomes from the nuclei, stain debris, and the background. Fig. 6.8 shows the input image (a), the thresholded...
Interestingly, the images show that while RCPs are clearly overlapping with the nuclear stain during interphase, the RCPs are enriched around the metaphase plate in cells in mitosis, when the nuclear membrane collapses ahead of cell division, which has previously also been demonstrated for the ...
DNA ploidy provides important information for the evaluation of the prognosis of prostate cancer. For the purpose of DNA cytometry and nuclear measurements... T Irinopoulou,J Vassy,M Beil,... - 《Cytometry》 被引量: 152发表: 1997年 A system for fluorescence metaphase finding and scoring of ...
To evaluate the involvement of a protein in cohesin regulation, preparations of metaphase chromosome spreads and classifications of chromosome shapes after depletion of the target protein are commonly employed." 关键词: Tokyo Japan Asia Cell Nucleus Cell Nucleus Structures Cellular Structures Chromatids ...
The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spi...
The cell cycle stage prediction is represented as probability distributions over 9 classes, namely: “Early G1”, “Late G1”, “S/G2”, “Metaphase”, “Anaphase”, “Telophase”, “Abberant”, “Over_seg”, and “Anaphase_defect”. Meanwhile, the protein localization is represented as ...
Cytogenetic analysis of Polyodon spathula匙吻鲟的细胞遗传学分析 Metaphase chromosome specimens of Polyodon spathula, injected with PHA and colchicin, were prepared from short-term culture of kidney cells with air-drying... XUE ShuQun,YIN HongBin,薛淑群,... - 《水生生物学报》 被引量: 0发表: ...
MINS accurately detects nuclei with condensed chromosomes or dividing nuclei during metaphase (Figure S1, yellow arrowhead). However, MINS does not distinguish these nuclei from interphase nuclei during the detection process; to this end, an increased signal intensity of these nuclei correctly reflects ...