The tungsten lamp has been used for many years as a light source for microscopy. Mercury, xenon and metal﹉alide lamps require control transformers to provide sufficient power to initiate an arc and also to maintain a stable current when the lamp is operating. The classical way of altering or...
Microscopy illumination systems can be overlooked when configuring a microscope setup; CoolLED designs and manufactures cutting edge LED Systems.
Inspection microscope users can obtain information from this article which helps them choose the optimal microscope illumination or lighting system for inspection of parts or components.
CoolLED pE-300ultra LED light for microscope offers fast controllable illumination for high speed microscopy applications, optogenetics & electrophysiology.
4). Next, we implemented NIR co-illumination on a Nikon Eclipse Ti wide-field fluorescence microscope. A straightforward modification of the epifluorescence illuminator to integrate an 885-nm laser diode enabled us to achieve an NIR co-illumination of 0.8 kW/cm2 over the same field as the...
Three-dimensional structured illumination microscopy (3D-SIM) doubles the spatial resolution along all dimensions and is used widely in cellular imaging. However, its temporal resolution is constrained by the need for sequential plane-by-plane movement of the sample using a piezo stage for imaging, ...
Barbara Foster is president and chief strategic consultant of TheMicroscopy& Imaging Place Inc. and Microscopy/Microscopy Education (MME) in McKinney, Texas; e-mail: bfoster@the-mip.com. References 1. J. Beacher (February 2011). Microscope illumination: the LED revolution.The Biomedical Scientist...
of structured illumination patterns were reviewed.As an example,a SIM system based on DMDmodulation and LED-illumination was demonstrated.A lateral resolution of 90 nm was achieved with gold nanoparticles.The optical sectioning capability of the microscope was demonstrated with Golgi-stained mouse brain...
Perhaps one of the most misunderstood and often neglected concepts in optical microscopy is proper configuration of the microscope with regards to illumination, which is a critical parameter that must be fulfilled in order to achieve optimum performance. The intensity and wavelength spectrum of light ...
The axial resolution of three-dimensional structured illumination microscopy (3D SIM) is limited to ∼300 nm. Here we present two distinct, complementary methods to improve axial resolution in 3D SIM with minimal or no modification to the optical sy