The present invention discloses a light source (10) for the illumination of microscopic specimens. The invention also discloses a scanning microscope system that possesses a light source (10). The light source (10) emits a combined light beam that is emitted by a first laser (4) and a ...
Illumination of specimens by darkfield requires blocking out of the central light rays along the optical axis of the microscope, which ordinarily pass through and around (surrounding) the specimen. Blocking these light rays allows only those oblique rays originating at large angles to strike the spec...
Fluorescence occurs either as autofluorescence of biological and inorganic structures or so-called secondary fluorescence after a treatment of the specimen with special dyes (fluorochromes, fluorescent markers) [1-3]. To perform fluorescence in a microscope the following requirements have to be met: ...
of structured illumination patterns were reviewed.As an example,a SIM system based on DMDmodulation and LED-illumination was demonstrated.A lateral resolution of 90 nm was achieved with gold nanoparticles.The optical sectioning capability of the microscope was demonstrated with Golgi-stained mouse brain...
A lateral resolution of 90 nm was achieved with gold nanoparticles. The optical sectioning capability of the microscope was demonstrated with Golgi-stained mouse brain neurons,and the sectioning strength of 930 nm was obtained. 展开 关键词: 光学显微镜 光学切片 超分辨率 结构照明 SIM卡 金纳米粒子 ...
This way, the complete phase structure of the image can be constructed with super-resolution approaching 2. We now ask whether 2km is the limiting spatial frequency of SIM. If the modulation grating is projected through the microscope condenser onto the object, then kg=km is indeed the largest...
of optical microscopy is limited by the wave diffraction nature, which functions as a low-pass filter during the imaging process. As a result, a sample’s fine structure (high-frequency component) cannot pass through the microscope system. However, a Moiré fringe with fine structure information...
The Leica LED3000 MCI™ for routine stereo microscopesprovides unique multi contrast illuminationto identify hard-to-find details on your samples or specimens. Four LED spotlights are arrangedin two arcs and inducedifferent, repeatable contrastfrom 2 different directions and angles. Through this ...
A microscope illumination optical system for an ultra-low magnification wherein the roughness of a light diffusing plate is not visible, and uniform illumination can be performed, and wherein specimens of weak contrast, a phase distribution, etc. can be made visible through adjustment of the numer...
Microscope - Magnification, Optics, Illumination: The limitations on resolution (and therefore magnifying power) imposed by the constraints of a simple microscope can be overcome by the use of a compound microscope, in which the image is relayed by two l