In order to acquire clear images when different positions on a specimen in the direction of the optical axis of an illumination optical system are observed with a sheet illumination method, a microscope apparatus of the present invention includes: a detection optical system that detects fluorescence ...
Microscopy illumination systems can often be overlooked when configuring a microscope setup – whether that is for widefield fluorescence or transmitted light applications. However, the type and amount of light hitting the sample plane will make or break an experiment. While microscopy illumination syste...
A illumination device for microscope, which has a illumination axis, includes illumination unit, which emits rays of illumination light, for illuminating a specimen, a field stop projection lens, which is located on the illumination axis between the illumination unit and specimen, a light deflector ...
Thus, it is estimated that further digitization advances in the field of observation by a microscope. [0006] However, a charge coupled device (CCD) used in a digital camera, or the like, is sensitive to a change in brightness, compared with a film used for the silver salt photography. ...
Structured illumination microscopy (SIM) has been a widely-used super-resolution (SR) fluorescence microscopy technique, but artifacts often appear in reconstructed SR images which reduce its fidelity and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM re...
Fluorescence occurs either as autofluorescence of biological and inorganic structures or so-called secondary fluorescence after a treatment of the specimen with special dyes (fluorochromes, fluorescent markers) [1-3]. To perform fluorescence in a microscope the following requirements have to be met: ...
If the modulation grating is projected through the microscope condenser onto the object, then kg=km is indeed the largest shift which can be projected since it uses the whole NA of the optical system (Figure 16.4A). But if the grating can be put in contact with the object, then there ...
The emitted fluorescence, captured by the same microscope objective, passes through a 60-nm-wide bandpass filter centered at 530 nm before impinging on the detector, a PMT. Shown in Figures 3a and 3d are two TPEF images acquired with the microscope operating in standard (linear scale) and AIM...
While it is tempting to adjust the intensity on these light sources with the rheostat, a better solution is the use of neutral density filters, often installed in the base of the microscope. For simpler systems or for a variable effect, place two polarizing filters covering the light port. ...
The invention relates to a microscope, in which a layer of the sample is illuminated by a thin strip of light (11) and the sample is viewed (5) perpendicular to the plane of the strip of light. The depth of the strip of light (11) thus essentially determines the depth of focus of ...