IL-21 and CD40L synergistically promote plasma cell differentiation through upregulation of Blimp- 1 in human B cells. J. Immunol. 190:1827-1836.Ding BB, Bi E, Chen H, Yu JJ, Ye BH (2013) IL-21 and CD40L synergistically promote plasma cell differentiation through upregu- lation of Blimp...
Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for30minutes at3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles. Cell culture supernates and other biological fluids- Remove particulates by...
Human IL-21 Proquantum Immunoassay Kit; 优势:灵敏度高;<0.3 pg/mL;定量范围宽:0.32-5000 pg/mL;样本用量少;Plasma 2-5 µL;Serum 2-5 µL;Supernatant 2-5 µL;时间:2小时;仪器:qPCR仪器; 原理如下: (A)Antibody-antigen binding: Antibodies bind to two separate epitopes on the antigen (d...
4 Depending on the stimulation context, IL-21 can be proapoptotic for B cells6, 7, 8 or can promote their differentiation to memory and plasma cells.7,9 IL-21 can also potently augment T-cell proliferation as a comitogen10 and cooperates with IL-7 or IL-15 to drive the proliferation ...
(1, 3 ‑ 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (9). IL-21 engagement of the IL‑21 receptor on B cells induces Blimp-1 (which mediates plasma cell differentiation), and is important for memory responses (...
(95% CI) Mann–WhitneyUtest.eThe delay in the rebound of plasma viremia was correlated against measures of SIV content, NK cell differentiation, and NK cell activity (as indicated at left;n = 13) from the final on-ART measurement (d339–374 p.i.) or during rebound following ATI ...
Serial dilutions of DNA from cell lysates of HIV-1-infected 293T cells (provided by Dr F. Bushman, University of Pennsylvania, USA) were used as references and positive controls. ELISA. Human IL-21 protein was determined in BLT mice plasma or culture supernatant using the human IL-21 ...
IL-21 is mainly produced by follicular helper T cells (Tfh), natural killer (NK) T cells, and T helper 17 (Th17) cells, which can enhance the function of macrophages, NK cells, and NKT cells, and promote the differentiation of B cells and plasma cell production, with broad regulatory ...
Should the same antigen enter the body again this circulating antibody acts quickly to destroy it, and at the same time memory cells quickly divide to produce new clones of the appropriate type of plasma cell. Clearly, then B cells and antibodies form a critical part of the human immune ...
. Like IL-2, IV-administered IL-21 had a plasma half life of only minutes with the main clearance in the kidney. Three hours after injection, the majority of the labeled material extracted from kidney was still retained in a Biomax 5K NMLW membrane (Millipore). Since it has previously ...