to the signal of Hsp60, used as a loading control from the same sample. Bands corresponding to phosphorylated proteins were normalized to the signal of the corresponding total protein which had been normalized to Hsp60. The results are displayed onSupplementary Figure 4. Apoptosis was further demo...
To demonstrate the usefulness of this pathway as a common target for the development of broad-spectrum antivirals, the inhibitory effect of drug compounds targeting endosomal membrane proteins were investigated. This study entailed direct comparison of drug effectiveness against animal and human pathogenic...
This can be summarized into eight key properties that are desirable in a human drug target, namely: high hydrophobicity, high length, SignalP motif present, no PEST motif, more than two N-glycosylated amino acids, not more than one O-glycosylated Ser, low pI and membrane location. The ...
Analysis of proteomes of biological samples used to identify and quantify either global or targeted proteins, typically through mass-spectrometry-based and gel-based methods. Xenobiotic A substance that is not endogenous to the environment or system. Rights and permissions Springer Nature or its licens...
Alterations in membrane proteins (MPs) and their regulated pathways have been established as cancer hallmarks and extensively targeted in clinical applications. However, the analysis of MP-interacting proteins and downstream pathways across human maligna
Generally, proteins are found at five major sites, namely, plasma membrane, outer membrane, extracellular region, cytoplasm, and periplasm. The localization of protein scan help to identify them as drug or vaccine targets: membrane proteins are targets for vaccines and cytoplasmic proteins are ...
The TGF-β superfamily contains over 30 different proteins, such as BMPs, Activins, Inhibins, and the TGF-β1 isoforms [1–3]. The pathway contributes to regulation of various cellular processes, such as apoptosis, cell differentiation, cell growth as well as tumor suppression and immune ...
(PTT) effect. HNP coated by red blood cell membrane loaded with DSPE-PEG-cRGD peptide for the therapeutic application of liver cancer greatly prolonged in vivo circulation time and enhanced local targeting efficacy as well as low toxicity; HNP coated by the murine macrophage cell membrane (RAWM...
et al. Screening and large-scale expression of membrane proteins in mammalian cells for structural studies. Nat. Protoc. 9, 2574–2585 (2014). Article CAS PubMed PubMed Central Google Scholar Kawate, T. & Gouaux, E. Fluorescence-detection size-exclusion chromatography for precrystallization ...
Endothelial extracellular vesicles contain protective proteins and rescue ischemia-reperfusion injury in a human heart-on-chip. Sci. Transl. Med. 12, eaax8005 (2020). Article Google Scholar Si, L. et al. A human-airway-on-a-chip for the rapid identification of candidate antiviral therapeutics...