In addition, CD68+ macrophages in human obese compared to lean AT showed increased expres- sion of proliferation markers, but it remained unclear whether this was associated with a specific macrophage subset16. Our data corroborate the concept that CD11c+ "M1" macrophages in human vAT originate ...
Macrophages (M1 or M2) were analyzed using FACSCanto II (BD Bioscience) flow cytometer, and data analyzed using FlowJo X Software. Macrophage incubation and LM metabololipidomics Macrophages were routinely incubated at 5 × 106 cells/ml of PBS with Ca2+ and Mg2+ (PBS + Ca/Mg)...
Together, these results suggest that human IRF1 governs IFN-γ-dependent macrophage activation and resistance to intracellular pathogens. Download: Download high-res image (2MB) Download: Download full-size image Figure S6. Response to IFN-γ in myeloid cells from P1 and P2, related to Figure 6...
Macrophage colony-stimulating factor (M-CSF) has well known effects on murine bone marrow,4,5 but it has been difficult to show any appreciable effect on the growth of colony-forming unit-macrophage (CFU-M) from human bone marrow.4, 5 Even with highly purified marrow progenitor cells, the...
The method involved embryoid body (EB) formation in hES media, directed differentiation with macrophage colony-stimulating factor and interleukin (IL)-3, and harvest of nonadherent monocytes from the culture supernatants. hES monocytes (esMCs) were analyzed by microscopy, flow cytometry, transcriptome...
flow cytometryHematopoietic stem cells are the source of all inflammatory cell types. Discovery of specific cell surface markers unique to human hematopoietic stem (HSC) and progenitor (HSPC) cell populations has facilitated studies of their development from stem cells to mature cells. The specific ...
[13]. Different tissue-specific macrophage populations have different transcriptional profiles and epigenetic markers, which are determined by tissue-specific factors [14]. Macrophages have multiple functions, including the recognition and clearance of pathogens, killing of target cells, antigen presentation...
a, Schematic of human germ cell development. Differentiation stages, key markers and stages covered by this study (yellow) are shown.b, Summary of the acronyms used in this study.c, Flow cytometric analysis of the expression of AG and DT or VT during BMP-driven M1-AGDTor F1-AGVThPGCLC ...
membrane, evidenced by lower levels of soluble TREM2 detected in the supernatant (Fig.2B). We validated macrophage identity after differentiation of the PLCγ2 Ko iPSC lines by detection of typical lineage markers by flow cytometry, which were expressed in all lines (Fig.2C). The three PLC...
Flow cytometry analysis showed that the cells could express some of the endothelial lineage markers, while they could also express CD14, which is considered a marker of monocyte/macrophage lineages throughout culture. In endothelial function assays, the cells demonstrated a lower level of expression ...