Flow cytometry analysis of F4/80 and CD11c (M1 pro-inflammatory) macrophage markers in mouse inguinal and perigonadal adipose depots.Danny GauvreauAbhishek GuptaAlexandre FisetteFunQun TomKatherine Cianflone
Although flow cytometry enables high throughput quantification of cell aggregates co-expressing macrophage and erythroblast markers, it cannot visually confirm whether the aggregates are genuine EBIs. While immunofluorescence microscopy allows visualization of EBIs, its low throughput limits its use for ...
应用 in vivo Monocyte/Macrophage depletion/Functional assays/Immunohistochemistry (paraffin)/Immunohistochemistry (frozen)/Flow cytometry 产品形式 PBS , pH 7.0 Contains no stabilizers or preservatives 内毒素水平 <2EU/mg (<0.002EU/μg)使用 LAL gel clotting 测定 纯度 >95% Determined by SDS-PAGE ...
m Representative immunofluorescence images of liver tissues stained with various cell markers including F4/80 (macrophage), CD26 (hepatocyte), CD31 (endothelial cell), Ck7 (cholangiocyte), and αSMA (aHSC). n Populations of cells were analyzed using a VECTRA tissue analyzer and the InForm 2.2....
Thymic dendritic cells are negative for the stem cell antigen-2 (Sca-2), the B cell-specific form of CD45 (B220), the mouse macrophage markers Fc receptor and F4/80, and the granulocyte marker Gr-1. However, although they do not express the T cell markers Thy-1, CD2, CD3, CD4 ...
Flow cytometry Winkler, I. G., et al. (2012). “Hematopoietic stem cell mobilizing agents G-CSF, cyclophosphamide or AMD3100 have distinct mechanisms of action on bone marrow HSC niches and bone formation.” Leukemia 26(7): 1594-1601 in vivo Monocyte/Macrophage depletion Bedoret, D., et ...
In the retrieved dataset the following populations were sorted based on the same markers as our flow cytometry analysis: Transitional 1, Transitional 2, Follicular and Marginal Zone. The following populations were considered equivalent between ImmGen and our flow cytometry analysis: Pro-B to FrB/C,...
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF) Isotype Rat IgG1, κ Ave. Rating Submit a Review Product Citations publications PMA/Ionomycin-stimulated (6hrs) C57BL/6 mouse splenocytes stained with XMG1.2 APC and B220 (...
16. We and others have reported that markers of cellular senescence and fibrogenesis increase within the ovarian stroma with aging11,12,15, although the specific cell types that become senescent and/or profibrotic remain unknown. In addition to increased senescence and fibrotic markers, the ovarian ...
f IMRCs’ expression of MSC-specific surface markers was determined by flow cytometry. Isotype control antibodies were used as controls for gating. Like MSCs, the IMRCs are CD34−/CD45−/HLA–DR−/CD90+/CD29+/CD73+/CD105+ cells. g Representative immunofluorescence staining of IMRCs ...