Macrophages (M1 or M2) were analyzed using FACSCanto II (BD Bioscience) flow cytometer, and data analyzed using FlowJo X Software. Macrophage incubation and LM metabololipidomics Macrophages were routinely incubated at 5 × 106 cells/ml of PBS with Ca2+ and Mg2+ (PBS + Ca/Mg)...
glioma, immunotherapy, lymphocyte, macrophage, microgliaObjectTo date, glioma immunotherapy has been focused mostly on stimulating antitumor peripheral lymphocyte responses; however, some data suggest that microglia and/or macrophages (not lymphocytes) are the predominant inflammatory cells infiltrating gliomas...
In a subset of patients, a more detailed flow cytometry analysis of Class I and II major histocompatibility complex, B7-1, B7-2, CD11c, and CD14 expression was performed. Macrophage-like cells in primary glioma cultures were similarly assessed.Operative samples were obtained from 9 newly ...
Our present results reiterate the necessity of cGAS and IFI16 to drive interferon responses in the human macrophage-like cell line THP-1 during infection with HIV, HSV-1 and CMV or by stimulation with synthetic DNA. More interestingly, we demonstrated that IFI16 is also necessary for initiating...
OBJECTIVE: Biologically significant amounts of two procoagulant molecules, phosphatidylserine (PS) and tissue factor (TF), are transported by monocyte/macrophage-derived microvesicles (MVs). Because cellular cholesterol accumulation is an important feature of atherosclerotic vascular disease, we now examined...
Together, these results suggest that human IRF1 governs IFN-γ-dependent macrophage activation and resistance to intracellular pathogens. Download: Download high-res image (2MB) Download: Download full-size image Figure S6. Response to IFN-γ in myeloid cells from P1 and P2, related to Figure 6...
Macrophage colony-stimulating factor (M-CSF) has well known effects on murine bone marrow,4,5 but it has been difficult to show any appreciable effect on the growth of colony-forming unit-macrophage (CFU-M) from human bone marrow.4, 5 Even with highly purified marrow progenitor cells, the...
Thus, we established a novel, gentle flow-through method to isolate pure human umbilical endothelial cells from first trimester (FTUEC). FTUEC were characterized and their phenotype was compared to the umbilical endothelium in situ as well as to other fetal endothelial cell models from term of ...
To further define the multipotency of JNK-IN-8-expanded cells, we performed the colony-forming unit (CFU) assay. We found that JNK-IN-8 can also increase the colony-forming unit of granulocyte, erythrocyte, macrophage, and megakaryocyte (CFU-GEMM) by about four folds, on day 10 (Fig.2...
Given the impressive in vivo functionality of iPSC-Mac in the acute infection model, a potentially important extension of this work would be to evaluate the therapeutic efficacy of a macrophage-based immunotherapy in chronic infections withP. aeruginosa, as frequently observed in patients suffering fro...