Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets ...
Cytostasis and cytotoxicity as well as survival and growth of EMT6 cells following exposure to tumoricidal macrophages have been examined using multiparameter flow cytometry. Macrophages were resolved from tumor cells, and the number of surviving EMT6 cells at different interaction times was determined ...
Immunohistochemical stains showed colocalization of CCL2 with HRV in epithelial cells and CD68-positive macrophages, and flow cytometry showed increased CCL2(+), CD11b(+) cells in the lungs of OVA-treated, HRV-infected mice. Compared with lung macrophages from naive mice, macrophages from OVA-...
FACS and flow cytometry For cultured cells, cells were trypsinized, pelleted, and resuspended in 2% bovine serum albumin (BSA)/PBS as a single cell suspension for FACS sorting. Tissues from tdTomato MDA-MB-231 SORE6>GFP or tdTomato MDA-MB-231 minCMV>GFP primary tumors were washed once ...
As such, with the help of high-dimensional approaches, such as flow cytometry, mass cytometry and single-cell RNA sequencing (scRNA-seq), the precise phenotypes and transcriptomic programs of border-associated macrophages in the meningeal-choroid plexus-perivascular space were revealed. Our ...
To investigate cycle-related changes in macrophage phenotype, mammary gland cells from naturally cycling Cfms-Gfp mice recovered at estrus, metestrus, diestrus, and proestrus were analyzed by flow cytometry. Macrophage expression of MHCII was highest in the proestrus phase, with a 1.6-fold increase...
Verification of the differentiation process through flow cytometry, identifying key surface markers such as CD86, CD163, and CD206 to distinguish macrophage phenotypes Combine cell phenotype with functional assays and cytokine measurements indicative of M1 and M2 types ...
The induction of the inflammatory response and the recruitment of monocytes/macrophages were evaluated by immunofluorescence, flow cytometry and transwell assays. The expression of inflammatory cytokines was examined by RT–PCR and ELISA, and the signalling pathways involved were examined by western blot ...
Flow cytometry, H&E staining, quantitative real-time PCR, immunofluorescence, glucose uptake and output assays, confocal microscopy imaging, western blotting, GTTs and ITTs were conducted to investigate tissue inflammation, mitochondrial function and insulin resistance in vitro and in vivo. The roles ...
(Extended Data Fig.2). As flow cytometry antibodies were not available for the candidate genes, we used PrimeFlow to measure the dynamics of mRNA expression and detected increased levels of all three genes (ETS2,BRWD1andPSMG1) after TPP stimulation of unedited monocytes (Fig.1e). Deletion ...