Here, we used human immunoglobulin gamma 1 (IgG1) transgenic mice (named "hIgG1 transgenic mice" or "TG"), which are immunologically tolerant to human IgG1, to study the immunogenicity of 13 conventional antibodies and 2 CitAbs. We found that tolerance to non-germline encoded Ids is ...
to human FcRn transgenic mice (Tg32), preloaded with pooled human IgG (IVIg) to introduce natural competition for receptor binding (Fig. 1m), which is important as these mice have low levels of endogenous mouse IgG due to lack of binding to the human receptor30,31,32. This revealed ...
We describe a novel strain of human immunoglobulin transgenic mice and the use of this strain to generate multiple high-avidity human sequence IgG Mabs directed against a human antigen. The light chain transgene is derived in part from a yeast artificial chromosome clone that includes nearly half ...
Interestingly, anti-collagen-II antibodies were elevated in PIA in the susceptible mice. In the CIA model, the highly susceptible transgenic mouse had IgG subclass levels equivalent to the unaffected and disease resistant C57BL/6 mouse strain implying that the FcγRIIa lowers the threshold of IgG...
However, results obtained in this study demonstrated anti-tumor activity of hIgG1 TA99 in FcγRIIB knockout mice and in human FcγRI transgenic mice. These results emphasize the need for testing hIgG mAb in mice with functional human FcγRs. 展开 ...
totality or a portion of the transgene that comprises the gene Cγ of a G-class human immunoglobulin including at least the exon coding for the CH3 domain and the membrane exons m1 and m2; the invention also relates to the applications thereof in the production of IgG-class humanised ...
FcR-null (γ chain KO), FcγR-humanized, hFcRIIA/IIB transgenic, and hFcRIIIA/IIIB-transgenic mice were inoculated IV with 5*105 B16-FUT3 tumor cells, 100 ug of anti-sLeA Abs (5B1-hIgG1-GAALIE with G236A/A330L/I332E mutations) or isotype-matched control Abs were administered ...
Using five-feature mice that harbour YAC-based germline-configuration human IgM, Ig and Ig transloci in a mouse background disrupted for endogenous mouse IgH and Ig expression, gp140-specific human IgM mAbs were readily elicited following serial immunisation. These mAbs were converted to human IgG...
Transgenic mice harbouring a loss of function GPR10 variant found in an individual with obesity, gain excessive weight due to decreased energy expenditure rather than increased food intake. This evidence supports a role for GPR10 in human energy homeostasis. Therapeutic targeting of GPR10 may ...
and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis...