Production of transgenic mice - Hogan, Beddington, et al. - 1994 () Citation Context ...ed using a DNA purification kit (no. 28304; Qiagen). For the derivation of transgenic mice, DNA was microinjected into the male pronucleus of F 1 embryos (CBA � C57BL/6) according to standard ...
Leibo SP, De Mayo FJ, O'Malley B. 1991. Production of transgenic mice from cryopreserved fertilized ova. Mol Reprod Dev 30:313-319.Leibo SP, DeMayo FJ, O'Malley B. Production of transgenic mice from cryopreserved fertilized ova. Mol Reprod Dev 1991;30:313-9....
Production of Human Antibodies from Transgenic Mice来源: 互联网 关键词: production human antibodies Mice transgenic for human immunoglobulin genes offer the opportunity to investigators to derive fully human antibodies using well-established hybridoma technology. Several different strains of such mice are ...
Production of transgenic mice Standard molecular techniques were used to assemble the transgenic construct, pTRE-E2. This transgene contains the tetracycline responsive hCMV*-1 promoter [consisting of the tetracycline responsive element (TRE) and a minimal hCMV promoter] [25] from pTRE2 (Clontech ...
These results show that utilisation of human Ig genomic segments does occur in transgenic mice, paving the way for the derivation of a mouse strain that makes authentic human antibodies from inserted heavy and light chain gene loci. 展开
In M54 mice transgenic for a completely rearranged μa heavy chain there is a decrease in total B cells and the rearrangement of endogenous immunoglobulin genes is partially inhibited. Surprisingly, however, endogenous immunoglobulin gene rearrangement and significant heavy chain polypeptide production...
Tumours producing regulatory peptides or amines common to the nervous and endocrine systems are called neuroendocrine tumours [1, 2]. They are composed of variably differentiated cells that share a number of morphologic markers normally expressed by neurons and endocrine cells [3, 4]. In laboratory...
Production of human lactoferrin in the milk of transgenic mice and the generation of transgenic cows harbouring a transgene encoding human lactoferrin: H. de Boer, W. Eyestone, F. Pieper, R. Strijker, J. Nuijens, S. He Lee and P. Krimpenfort. Gene Pharming Europe B.V., Leiden, ...
Such NT-ES cells can be used not only for experimental models of human therapeutic cloning but also as a backup of the donor cell's genome. Our most recent protocols for mouse cloning, as described here, will allow the production of cloned mice in ≥3 months. This is a preview of ...
The expression of chimeric genes in the mammary gland of transgenic farm animals has become an alternative for the large-scale production of recombinant proteins and for the modification of milk composition. In this paper, we show that a mouse/human chimeric antibody against the human CD6 leukocyt...