cultures of CD34+ human cord blood (CB) cells. After one week, ~40% of the cultured cells express CD34, indicative of >10-fold expansion of input CD34+ CB cells. This expansion may be further increased with the addition of small molecules such as UM729. See data tab for more details...
ex vivo expansion, CD34+ cells, stromal cell line, Fit3 ligand, thrombopoietinTo elucidate the effect of gene transfected marrow stromal cell on expansion of human cord blood CD34 + cells, a culture system was established in which FL and TPO genes were transfected into human stromal cell line...
as a result, diabetic wounds either do not heal or heal very slowly1. Efforts have been made to develop more effective therapeutics to cure wounds through tissue regeneration. We previously demonstrated that systemic administration of nanofiber-expanded CD34+cells accelerated acute ...
the in vitro expansion of HSCs remains a challenge due to incomplete understanding of the signaling networks underlying HSC self-renewal. Here, we show that culturing human cord blood (CB) CD34+cells with JNK-IN-8, an inhibitor of
During the rapid expansion period, the primary CD34+ stem cells exhibited representative spherical morphology and were suspended in the culture medium (Fig. 1a). Flow cytometric analyses showed that they were highly positive for CD34 (Fig. 1b). Then CD34+ stem cells were transfected with lentivir...
We have recently found that miR-17 is necessary in the cell-extrinsic control of cord blood (CB) CD34(+) cell function. Here, we demonstrated that the proper level of miR-17 is also necessary in the cell-intrinsic control of the hematopoietic properties of CB CD34(+) cells.The miR-17 ...
De Bruyn, C., Delforge, A., Martiat, P., Bron, D.Ex vivo expansion of megakaryocyte progenitor cells: cord blood versus mobilized peripheral blood.Stem Cells Dev.14(4), 415-424 (2005). Nimgaonkar, M. T., et al.A unique population of CD34+ cells in cord blood.Stem ce...
EX-VIVO EXPANSION OF HUMAN BONE-MARROW AND CORD-BLOOD CD34+ PROGENITOR CELLS S Heimfeld,R Fei,J Tsui,... - 《Blood》 被引量: 0发表: 1993年 Massive ex vivo generation of functional dendritic cells from mobilized CD34+ blood progenitors for anticancer therapy We report that blood cell ...
We show a robust oncogenic cooperativity between activated forms of c-KIT and REtr in human CD34+ progenitor cells using a recently published ex vivo progenitor cell expansion assay.5, 12, 13 Together with REtr, c-KIT(N822K) expression blocks cellular differentiation of progenitor cells at the ...
Arrows highlight cells of interest. Scale bars: main, 100 μm; expansion, 50 μm. d, UMAP of stromal cell types across development. The dotted line highlights key lineages. e, Spatial mapping of cell types from the scRNA-seq data to spatial transcriptomics data of 17 PCW terminal ileum ...