Characterization of retroviral gene transfer into highly purified human CD34(-) cells with primitive hematopoietic capacity. Mol Ther. 2002;5:635-643.Murdoch B, Gallacher L, Chadwick K, Bhatia M (2002) Characterization of retroviral gene transfer into highly purified human CD34(−) cells with ...
CD34+ cells, present at a frequency of 0.18 卤 0.052% among leukocytes from peripheral blood (PB), can be rapidly and efficiently en- riched to a frequency of 38.6-87.1% (&4*4 by high-gradient magnetic cell separation (MACS) for Immunopheno- typing, characterization in colony-forming cell...
the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired human CD34+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD34 antigen is expressed on hematopoietic stem and progenitor cells. ...
cells, especially for hematopoietic and endothelial stem cells. CD34+ stem cells are multipotent and can differentiate to all hematopoietic cell types in blood. CD34+ cells can also give rise to all lymphohematopoietic lineages even though they comprise only a small percentage of the cell population...
Recent evidence indicates that human hematopoietic stem cell properties can be found among cells lacking CD34 and lineage commitment markers (CD34−Lin−). A major barrier in the further characterization of human CD34− stem cells is the inability to detect this population using in vitro assays...
cells, especially for hematopoietic and endothelial stem cells. CD34+ stem cells are multipotent and can differentiate to all hematopoietic cell types in blood. CD34+ cells can also give rise to all lymphohematopoietic lineages even though they comprise only a small percentage of the cell population...
After a 7-day suspension culture of CD34+ cells with these 5 CSFs, CD34+ cells expanded 14.5-fold, and CD34+/CD33− cells and CD34+/CD33+ cells were also expanded 2.9-fold and 307-fold, respectively. Most secondary colonies derived from expanded cells were small; however, the absolute ...
Despite advances in diabetic wound care, the significant number of amputations that occur every year demands more effective therapeutics. Herein, we offer an aminated polyethersulfone nanofiber-expanded human umbilical cord blood-derived CD34+ cells (hen
The cells were passaged reg- ularly when they reached [80 % confluency. The purity and cell viability was assessed by May–Gru¨nwald–Giem- sa stain method and 0.4 % trypan blue, respectively. Characterization of cultured cells The CD34? cells were characterized by performing ...
In this work, we report conditions for basal and high yield in vitro expansion of a CD133+/CD34+ subset of progenitor cells from human UCB stimulated by estradiol, a hormone involved in both normal cell proliferation and development of cancer. A detailed molecular characterization of such process...