Results The expression of myeloid and lymphoid specific markers was increased and decreased in AWJ and FBS containing culture medium, respectively. Conclusion This study provided evidence for the differentiation of UCB-derived CD34 cells toward myeloid lineage and lack of their differentiation toward ...
CD34 + cells+DNA 66,9% FL-2 F L - 3 CD34 F L - 4 ABCDE H-2K K C D 3 4 CD34 + cells-DNA 0,9% CD3 + Tcells-DNA Freshperipheralbloodmononuclearcells(PBMC)partiallyenrichedforCD34 + cellsweretransfectedbyNucleofection®using
An anti-CD40L monoclonal antibody (hu5C8) and/or CTLA4-Ig molecule were used in primary mixed lymphocyte culture (MLC) with irradiated CD34+ blood cells and allogeneic responders. Then, secondary MLC, cytotoxic activity, and effector cytokine expression and production were measured. Results Each re...
Human CD34+cells from different sources disclose a specific stemness signature The "stemness" signature of cells dislodged from their niche by the drug is attenuated by the combined use with G-CSF, which emphasizes the gene expression profile induced by G-CSF treatment. These data indicate that...
Table 1.HSC Expansion Culture with CD34+Human Cord Blood Cells Cultured in StemSpan™ SFEM Containing CD34+Expansion Supplement Shown are the percent CD34+cells, fold expansion of total nucleated cells (TNC) and CD34+cells, and numbers of colony-forming units (CFU) produced per input CD34+cell...
Conclusions: This study indicates that CD34 cells obtained from dental pulp can be used for engineering bone, without the need for prior culture expanding procedures. Using autologous stem cells, this schedule could be used to provide the basis for bone regenerative surgery...
Eventually, the percentage of CD1a+ cells decreased. However, typical CD1a+ DCs still emerged in culture of sorted day-12 CD13hiCD1a- cells, and adding interleukin-4 to bulk cultures at that time led to the persistence of the CD1a+ population while diminishing CD14 expression. Thus, this...
We identified from peripheral blood a population lacking the expression of CD34, lineage markers, CD45 and CD133 (CD34−Lin−CD45−CD133− cells), endowed with the ability to differentiate after a 6-week culture into both hematopoietic and endothelial lineages. The bilineage potential of ...
Recent advances in the ability to purify and culture hematopoietic cells with the CD34+CD38- phenotype and with in vivo repopulating potential from human umbilical cord blood provide a direct means of testing the ability of transfer vectors to transduce these cells. Here we demonstrate the efficient...
Human ESC-derived CD34+ cells (n = 5) were isolated from coculture on day 11 using magnetic bead based separation method. CD34 microbeads were used to select CD34+ cells positively after depleting CD14+ and CD73+ cells. For comparison, CD34+ cells isolated from BM (n = 6) were used. ...