CD34Mesenchymal stem cellsAcellular Wharton's jellyMyeloid lineage differentiationLymphoid lineage differentiationIntroduction Hematopoietic stem cell transplantation (HSCT) is a proper treatment for many cancers, including malignant and non-malignant hematologic and immune disorders. However, due to the small ...
the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired human CD34+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. The CD34 antigen is expressed on hematopoietic stem and progenitor cells. ...
CD34 CD38 and CD34 HLA-DR cells in BM stem cell grafts correlate with short-term engraftment but have no influence on long-term hematopoietic reconstitution after autologous transplantation 热度: Amaxa®HumanCD34 + CellNucleoector®Kit ForHumanCD34 ...
recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state. The CD34+ Progenitor Cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. Therefore, CD34+ Progenitor Cells are suitable for a series of ...
Generation of CD34+ humanized mouse models Stem cell research overview Pluripotent stem cells Hematopoietic stem cells Mesenchymal stem cells Oncology overview Tumor cell culture Tumor tissue storage and dissociation Tumor infiltrating leukocytes Tumor cell isolation EV isolation and analysis GMP-engineered T...
Co-culture of CD34+cells and dermal fibroblast Primary human dermal fibroblasts were co-cultured with CD34+cells in a well of a 6-well plate using fibroblast culture media. Dermal fibroblasts were mostly seeded at 3 × 105cells/well in a 6-well plate and cultured them in DMEM media fo...
CD34+ human marrow cells not expressing T cell-, B cell-, and myeloid cell-associated antigens (TBM-) were cloned by two-color cell sorting into culture wells containing irradiated marrow stromal cells. After 4 wk of culture, 3.7 +/- 2.1% of these cells generated colony-forming cells (CF...
Regulatory T cells (Tregs) Monocytes and Mo-DCs Generation of CD34+ humanized mouse models Stem cell research overview Pluripotent stem cells Hematopoietic stem cells Mesenchymal stem cells Oncology overview Tumor cell culture Tumor tissue storage and dissociation Tumor infiltrating leukocytes Tumor cell is...
Each molecule was used separately, or in combination, in further MLC experiments where T-cell alloreactivity to CD34+ cells in the presence of costimulatory blockade was measured by 3H-thymidine incorporation assay after 6 days of culture (B). Inhibition of allogeneic T-cell response by anti-...
found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells. ...