Here we created a comprehensive molecular atlas of the human brain vasculature using single-cell RNA sequencing (scRNA-seq) analysis in the developing, adult/control and diseased human brain (Fig. 1a, Extended Data Fig. 1 and Supplementary Methods). We identified extensive heterogeneity among ECs ...
The human cortex comprises diverse cell types that emerge from an initially uniform neuroepithelium that gives rise to radial glia, the neural stem cells of the cortex. To characterize the earliest stages of human brain development, we performed single-c
with 11.3M cells or nuclei that covered nearly all major regions of the brain in health and diseases as well as 103 studies of mouse data of 15M cells. We demonstrate the utilities of the Brain Cell Atlas in the discovery of putative NPCs in adults...
Fig. 1: The reference infant brain atlas at 12 months. a,b, Transverse sections of T1w and T2w atlases and the T1w and T2w images of an individual subject. c, White and pial cortical surface atlases overlaid onto the corresponding tissue segmentation atlases. The cortical surfaces of the...
R. et al. Single-cell analyses identify brain mural cells expressing CD19 as potential off-tumor targets for CAR-T immunotherapies. Cell 183, 126–142 (2020). Article CAS PubMed PubMed Central Google Scholar Vanlandewijck, M. et al. A molecular atlas of cell types and zonation in the...
Here we perform massively parallel single-cell RNA sequencing (scRNA-seq) of human retinas using two independent platforms, and report the first single-cell transcriptomic atlas of the human retina. Using a multi-resolution network-based analysis, we identify all major retinal cell types, and ...
2c). No differences were found between independent control and C9 ALI-COs in the reconstructed path recapitulating the milestones of human brain development (Fig. 2c and Extended Data Fig. 2d). To confirm this finding, we projected transcriptomic data obtained from the organoid cell populations...
the brain region macroscale architecture mainly represented by gene expression analysis of individual cells, brain structures, and isolated cell populations1,2,3,4,5,6; and (ii) macroscale studies of the structural and functional organization of the brain mainly based on brain imaging techniques7,8...
The entire dataset is available on http://www.macrophages.com/hu-cell-atlas, where a webstart version of BioLayout Express3D enables visualisation of the average expression of each cluster, and the specific expression of individual genes across the dataset. Additional file2: Table S2 provides ...
Fig. 3: A human methylation atlas of 205 samples across 39 cell type groups. a, A total of 953 genomic regions unmethylated in a cell-type-specific manner. Each cell in the plot marks the average methylation of one genomic region (column) at each of 39 cell types (rows). Up to 25...