Mesenchymal stem cells (MSCs) are a common kind of multipotent cell in vivo, but their heterogeneity limits their further applications. To identify MSC subpopulations and clarify their relationships, we performed cell mapping of bone-marrow-derived MSCs through single-cell RNA (scRNA) sequencing. In...
Here we show that transfer learning鈥攖he branch of machine learning that concerns passing information from one domain to another鈥攃an be used to efficiently map bone marrow biology between species, using data obtained from single-cell RNA sequencing. We first trained a multiclass logistic ...
Decoding lymphomyeloid divergence and immune hyporesponsiveness in G-CSF-primed human bone marrow by single-cell RNA-seq Guoju You, Man Zhang, Zhilei Bian, Huidong Guo, Zhengyang Xu, Yanli Ni, Yu Lan, Wen Yue, Yandong Gong, Yingjun Chang, Xiaojun Huang & Bing Liu Cell ...
Single-cell RNA sequencing analysis of human bone-marrow-derived mesenchymal stem cells and functional subpopulation identification Article Open access 01 April 2022 Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties Artic...
The development of single-cell and spatial transcriptomics methods was instrumental in the conception of the Human Cell Atlas initiative, which aims to generate an integrated map of all cells across the human body. These technology advances are bringing
Human T cells coordinate adaptive immunity in diverse anatomic compartments through production of cytokines and effector molecules, but it is unclear how tissue site influences T cell persistence and function. Here, we use single cell RNA-sequencing (scRNA-seq) to define the heterogeneity of human ...
Here, we dissect the diversity of LrICs by a comprehensive transcriptomic profiling using the unbiased single-cell RNA-sequencing (scRNA-seq). A total of 70, 706 of CD45immune cells from the paired liver perfusion, spleen and peripheral blood as references were profiled. We identified more ...
Here we applied single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq) to over 8,000 human immunophenotypic blood cells from fetal liver and bone marrow. We inferred their differentiation trajectory and identified three highly ...
Single-cell RNA-seq with waterfall reveals molecular cascades underlying adult neurogenesis. Cell Stem Cell 17, 360–372 (2015) Article CAS PubMed PubMed Central Google Scholar Macosko, E. Z. et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. ...
在本周一的文章(复现原文(一):Single-cell RNA sequencing of human kidney(step by step))中,我们完成了scRNA-seq数据的质控(Hemberg-lab单细胞转录组数据分析(三)- 原始数据质控)、批次校正、找Marker基因(单细胞分群后,怎么找到Marker基因定义每一类群?)、UMAP可视化和tSNE可视化(Hemberg-lab单细胞转录组数据分...