IMPORTANT: For cells that arrive in a shipping flask please refer to the enclosed 'cell recovery procedure'. Cell Line Stability Cells will undergo genotypic changes resulting in reduced responsiveness over time in normal cell culture conditions. Genetic instability is a biological phenomenon that ...
The ability of transducted cell lines to adhere to extracellular matrix (ECM) proteins was assessed using the CytoSelect™ 48-Well Cell Adhesion Assay (Cell Biolabs, Inc) according to the manufacturer's protocol. The ability of the cells to adhere to fibronectin, collagen I, collagen IV, ...
Abstract Introduction Protocol Results Discussion Disclosures Acknowledgements Materials References English Automatically Generated Xia, J., Gill, E. E., Hancock, R. E.NetworkAnalyst for statistical, visual and network-based meta-analysis of gene expression data.Nature Protocols.10(6), 823-844 (2015)...
Normal mouse fibroblast (3T3) and human colon cancer (HT29) cell lines were procured from the American Type Culture Collection (ATCC). Cells were grown and maintained in Roswell Park Memorial Institute-1640 (RPMI) media added with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (P...
The cell pellet was resuspended in PBS (150 µL) and diluted to 1:10 before being assayed according to the manufacturer’s protocol. 2.8.2. Catalase Enzyme activity of catalase was measured according to the method described in Kjær et al. [31]. The cell pellet was resuspended in ...
Total DNA content of the fractions was related to the arbitrary light unit and plotted as test over control (T/C ([%]) Single‑cell gel electrophoresis (comet assay) Comet assay was performed following the protocol of Gedik and co-workers (1998). Therefore, 500,000 HT29 cells were ...
Therefore, the toxicity value obtained using a monoculture of enterocyte-like cells, i.e., Caco-2 cells, was compared to a co-culture of Caco-2 cells and HT29-MTX, the mucus-producing cells. To compare the two in vitro cell models, 19 cytotoxic B. cereus supernatants were tested in ...
Proteome Profiler Human Protease Array Kit (R&D System, ARY021) was used according the manufacturer's procedure for the screening of changes in proteases expression level in the cell culture supernatants and lysates. The control-mouse rabbit anti-β-actin antibody conjugated with HRP was obtained ...