2.Cell thawing: 1.Warm up the medium, spray with 70% ethanol and transfer into the hood. 2.Take a bullet of cells from freezer and immediately immerse into 37 °C water bath and stir until the cells are thawed. Spay with 70% ethanol and dry. 3.Open the bullet in the hood and fla...
Cell culture and transfection HEK293T cells were cultured in high glucose Dulbecco's modified Eagle's medium (Gibco, Gaithersburg, MD, USA) supplemented with 10% (v/v) fetal bovine serum (Gibco), 100 units penicillin/ml, 100 μg streptomycin/ml (Sigma-Aldrich, Arklow, Ireland) and incubat...
The expressed cBD-1 protein isolated from cell culture medium was detected by Western-blot suggesting that cBD-1 gene could be secretively expressed in eukaryotic cells. These results also provide a basis for further investigation of canine 尾-defensin function [Acta Zoologica Sinica 54(5): 897-...
The HEK-293T cell cultured by the invention has stable growth state and good dispersion, and is suitable for suspension culture in serum-free medium and chemical composition. 全部详细技术资料下载 【技术实现步骤摘要】 —种HEK-293T细胞的悬浮驯化和无血清驯化方法本专利技术涉及一种HEK-293T细胞的...
Cell culture studies Human HEK293T cells (ATCC-CRL-3216) were maintained under standard culture conditions with Dulbecco's modified Eagle's medium (DMEM, Gbico #C11995500BT) supplemented with 10 % fetal bovine serum (FBS, Gbico #10270106) and 1 % penicillin/streptomycin in a 5 % CO2air atm...
The luciferase assay working solution component (A + B) was added directly to the culture medium using an equal volume to the volume of the culture medium, which was 50 µL. The cells were incubated for 30 min while gently rocking at room temperature. After that, luminescence was ...
过表达EoRPL11下调HEK293T细胞中蛋白质的翻译活性_英文_
Cell Culture HEK293T cells (CRL-3216, ATCC, Manassas, VA, USA) and HEK293 cells (catalog no. 103, NIH AIDS Reagent Program, Germantown, MD, USA) were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing high glucose (4.5 g/L), 2 mM l-glutamine, 10% heat-inactivated fet...
Medium was replaced every 2–3 days before patch clamping at ~27–42 days post cell seeding. Immunofluorescence staining For immunofluorescence staining (IF) cardiac aggregates derived by hiPSC differentiation in suspension culture were cryo-protected by incubation in saccharose solution (30% wt/vol ...