b One-step growth curve of HSV-1 in BV2 cells. HSV-1 infection infects BV2 cells with MOI = 1. c, d Protein levels of GM130, TLR3, and HSV-1 are analyzed semiquantitatively by scanning a grayscale, and βactin is used as internal parameter correction. The expression levels ...
Scale bar, 100 mm. All data are from representative experiments repeated 2–4 times. 4 MARCO 2.5 MARCO 3 2 1 KD = 3.0x10−9 Bmax = 3.1x10−9 0 0 20 40 60 Total gCΔC5/P added (nM) 2.0 1.5 1.0 0.5 r 2= 0.98 0.0 0123 1/Total gCΔC5/P added (nM) Figure 4 | MARCO ...
Mouse polyclonal anti-IgG Proteintech Cat# B900620; RRID: AB_2883054 Rabbit monoclonal anti-N6-Methyladenosine Cell Signaling Technology Cat# 56593;RRID: AB_2799515 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076;RRID: AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell ...
Analysis HSV-1 seropositivity Sera that had been banked at − 80 °C were used for serological analysis; serum HSV1-IgG titers were measured using commercially available enzyme immunoassays (ELISA) (IBL International, Hamburg, Germany) in accordance with the manufactures instructions. The assay...
3,34 Therefore, we examined the anti-HSV- 1 isotypic antibody response. Indeed. in the studies presented, gD(5-23) coupled to KLH induced IgGI and IgG2a antibodies without providing neutralising activity. Cellular anti-HSV-l mechanisms may be import ant for protection. Reliable protection ...
(C) Morphology of HSV-1-infected SpCas9 or SaCas9 cell lines 3 days after infection with HSV-1 (MOI of 0.1). White arrows indicate the cytopathic effect (CPE). Scale bar, 30 μm. (D) Immunostaining of ICP5 in HSV-1-infected SpCas9 or SaCas9 cell lines 3 days after infection ...
Fast-replicating neurotropic herpesviruses exemplified by herpes simplex virus-1 (HSV-1) naturally infect the central nervous system (CNS). However, most individuals intrinsically suppress the virus during a primary infection and preclude it from signifi
Corneal transparency is an essential characteristic necessary for normal vision. In response to microbial infection, the integrity of the cornea can become compromised as a result of the inflammatory response and the ensuing tissue pathology including ne
The antibodies used were mouse anti-GFAP (1:400; Sigma-Aldrich; GA5), Rabbit anti-STING (1:100; IMGENEX, IMG-6485A); and secondary antibodies (1:300), Alexa Fluor 488 Donkey anti-mouse IgG (A21202) and Alexa Fluor 568 Donkey anti-rabbit IgG(A10042) from Life Technologies. RNA ...
(a) Vaginal washes were obtained 3 days after challenging, and HSV-2 specific IgA and IgG antibodies were determined by enzyme-linked immunosorbent assay (ELISA) using a dilution of 1:5. (b) Serum samples were obtained at days 3 and 7 after challenging, and HSV-2-specific IgG antibodies ...