HSV-2 Candidate Vaccine Virus Deleted in Glycoprotein D (螖gD-2) Elicits High-Titer IgG2 Antibodies with Antibody Dependent Cell Mediated Cytotoxicity (ADCC) Activity, Protects Mice from Skin and Vaginal Challenge with Cinical Isolates of HSV-1 and HSV-2, and Prevents the Establishment of ...
HSV-2 IgM alone was positive in three cases, HSV-2 IgG alone was positive in 26 cases and three had a positive HSV-2 IgM and IgG result. Addition of IgM testing increased rate of detecting seroconversion, 31.87%, when only IgG ELISA was used, to 35.16 % patients when IgM test was ...
To investigate the mechanism of Golgi matrix protein 130(GM130) regulating the antiviral immune response of TLR3 after herpes simplex virus type 1(HSV-1) infection of microglia cells. We explored the regulatory effects of berberine on the immune response
Figure 5a shows that the levels of IgA and IgG antibodies directed to HSV-2 in vaginal washes did not differ between mice vaccinated with or without SVF. Similar findings were observed when serum IgG antibodies were studied (Figure 5b). The analysis of the frequency of CD4 þ and CD8 ...
Herpes simplex virus type 2 (HSV-2) is a major cofactor of human immunodeficiency virus type 1 (HIV-1) sexual acquisition and transmission. In the present study, we investigated whether HIV-1 and HSV-2 may interact at the cellular level by forming HIV-1 hybrid virions pseudotyped with HSV...
Our results now suggest that the potent inhibitory of effects of these compounds may result from their capacity to block interactions with both HSPGs and MARCO. Thus, multiple lines of evidence show low affinity, high abundance (HSPGs) and high affinity, low abundance (MARCO) receptors are ...
Serological changes of Herpes Simplex Virus in infection [2] To cater to the increasingly diverse clinical requirements, Mindray has launched a series of HSV assays including HSV-1 IgG, HSV-2 IgG, HSV-1+2 IgG, and HSV-1+2 IgM. These assays demonstrate high levels of sensitivity and specif...
IgG、IFN-)及IL一2表达量,并通过MTT法对小鼠的脾淋巴细胞增值率进行检测结果1、构建重组质粒PMV-gBD一570转化大肠杆菌DH5a,经菌落PCR、双酶切以及测序鉴定正确,质粒构建成功。2、将构建成功的质粒电穿孔转化入耻垢分枝杆菌MS中,经菌落PCR及双酶切鉴定正确,质粒转化成功。3、SDS—PAGE结果显示,重组质粒可以在MS中正...
An aliquot was incubated with IgG (Abcam, ab2410) as a control to determine background binding. The beads were washed for 5 min at 4 °C with rotation, twice with Low-salt Buffer, once with High-salt Buffer, once with LiCl Buffer, twice with TE Buffer. Immunocomplexes were eluted...
IgG controls showed no signal, indicating the specificity of the assays (data not shown). Lanes 1 and 2: LAT TRE ChIP primers; lanes 3-6: ICP0 promoter primers. (B) Cells were cotransfected with pSG28 and with or without expression vectors of BRG1 or K785R specified in the figure....