Objective This study analyzed the gene expression profile of HSPC in MDS patients (MDS-HSPC) using single-cell sequencing (scRNA-seq) and explored the role of variable splicing factor Rbm17 in the pathogenesis of MDS. Design We used scRNA-seq, CUT&Tag, CRISPR/cas9, and FCM technologies, ...
. Single-cell RNA-sequencing (scRNA-Seq) was performed from the mononuclear cell fraction of E::R+ (n=3) and E:R- (n=3) CBs, and will be used to analyze the impact of the translocation on B-lymphopoiesis in cord blood. RESULTS. CB-HSPCs were isolated (CD34+ purity 70-90%) and...